氯通道阻断剂DIDS对过氧化氢诱导PC12细胞凋亡的影响  

Effect of chloride channel blocker DIDS on apoptosis of PC12 cells induced by hydrogen peroxide

作　　者：任京力[1] 林玲[2] 王雁梅[1] 王福青[2] 李超彦[2] 蔡智慧[2] 常全忠[2,3] 

机构地区：[1]漯河医学高等专科学校药学系药理学教研室,漯河462002 [2]漯河医学高等专科学校基础部生理学教研室,漯河462002 [3]遵义医学院珠海校区生理学教研室,珠海519041

出　　处：《郑州大学学报（医学版）》2015年第1期17-19,共3页Journal of Zhengzhou University(Medical Sciences)

基　　金：国家自然科学基金资助项目81160157

摘　　要：目的:探讨氯通道阻断剂DIDS抗PC12细胞凋亡的作用机制。方法:取处于对数生长期的PC12细胞,随机分为对照组、模型组(用400 nmol/L H2O2诱导细胞凋亡)和DIDS组(H2O2+DIDS),培养12 h后,用MTT法测定各组细胞的存活率,Western blot法观察凋亡相关蛋白Caspase-3和Bcl-2蛋白的表达情况,用流式细胞仪检测细胞凋亡率。结果:400 nmol/L H2O2可以明显诱导PC12细胞损伤,细胞存活率为(35.07±1.23)%;DIDS组细胞存活率明显高于模型组,且具有一定浓度依赖性(F=640.420,P<0.001)。与对照组比较,模型组细胞凋亡率和Caspase-3蛋白表达增加,Bcl-2蛋白表达降低(P<0.05);与模型组相比,DIDS组Caspase-3表达降低,Bcl-2蛋白表达增加,细胞凋亡率降低(P<0.05)。结论:DIDS可能通过抑制Caspase-3依赖性凋亡信号通路发挥抗凋亡作用。Aim： To investigate the mechanism of chloride channel blocker DIDS on apoptosis of PC12 cells.Methods： PC12 cells in logarithmic growth period were randomly divided into control group,model group（ induced by 400 nmol / L H2O2 for 12 h） and DIDS group（ induced by 400 nmol / L H2O2 and different doses of DIDS for 12 h）.The cell survival rate was measured using MTT,the expressions of apoptotic related proteins Caspase-3 and Bcl-2 were detected by Western blot,and apoptosis was observed by flow cytometry.Results： 400 nmol / L H2O2 could induce PC12 cell damage,and the cell survival rate was（ 35.07 ± 1.23） %.The survival rate of DIDS group was higher than that of the model group（ F = 640.420,P〈 0.001）.Compared with those of the control group,the apoptosis rate and the expression of Caspase-3were higher,and Bcl-2 expression was lower（ P〈 0.05）.Compared with those of the model group,the apoptosis rate and the expression of Caspase-3 were lower,and Bcl-2 expression was higher（ P〈 0.05）.Conclusion： DIDS might be antiapoptotic through inhibiting Caspase-3 dependent apoptotic signaling pathway.

关 键 词：DIDS PC12细胞 凋亡 CASPASE-3 BCL-2 

分 类 号：R338.2[医药卫生—人体生理学]