Molecular mapping of a stripe rust resistance gene in Chinese wheat cultivar Mianmai 41  

作　　者：REN Yong LI Sheng-rong WEI Yu-ming ZHOU Qiang DU Xiao-ying HE Yuan-jiang ZHENG You-liang 

机构地区：[1]Triticeae Research Institute, Sichuan Agricultural University [2]Mianyang Institute of Agricultural Science/Mianyang Wheat Experimental Station, China Agriculture Research System(CARS)

出　　处：《Journal of Integrative Agriculture》2015年第2期295-304,共10页农业科学学报（英文版）

基　　金：supported by the China Agriculture Research System(CARS03);the National Basic Research Program of China(2011CB100100)

摘　　要：Stripe rust, caused by Puccinia striiformis f. sp. tritici（Pst）, is one of the most damaging diseases of wheat. Chinese wheat cultivar Mianmai 41 showed high resistance against most of the prevailing Pst races in China. Genetic analysis of the F1, F2 and F2：3 populations from a cross between Mianmai 41 and a susceptible line Mingxian 169 indicated that resistance to Pst race CYR32 was conferred by a single dominant gene, temporarily designated as Yr MY41. Molecular marker analysis placed the gene on chromosome 1B near the centromere. Six co-dominant genomic SSR markers Xwmc329, Xwmc406, Xgwm18, Xgwm131, Xgwm413, and Xbarc312, and one STS marker Xwe173 linked with the resistance gene. The two closest flanking SSR markers were Xgwm18 and Xwmc406, with genetic distances of 2.0 and 4.9 c M, respectively. A seedling test with 29 Pst isolates indicated the reaction patterns of Mianmai 41 were different from those of lines carrying Yr3, Yr9, Yr10, Yr15, Yr26, and Yr CH42 on chromosome 1B. Allelic tests indicated that Yr MY41 is likely a new allele at Yr26 locus.Stripe rust, caused by Puccinia striiformis f. sp. tritici（Pst）, is one of the most damaging diseases of wheat. Chinese wheat cultivar Mianmai 41 showed high resistance against most of the prevailing Pst races in China. Genetic analysis of the F1, F2 and F2：3 populations from a cross between Mianmai 41 and a susceptible line Mingxian 169 indicated that resistance to Pst race CYR32 was conferred by a single dominant gene, temporarily designated as Yr MY41. Molecular marker analysis placed the gene on chromosome 1B near the centromere. Six co-dominant genomic SSR markers Xwmc329, Xwmc406, Xgwm18, Xgwm131, Xgwm413, and Xbarc312, and one STS marker Xwe173 linked with the resistance gene. The two closest flanking SSR markers were Xgwm18 and Xwmc406, with genetic distances of 2.0 and 4.9 c M, respectively. A seedling test with 29 Pst isolates indicated the reaction patterns of Mianmai 41 were different from those of lines carrying Yr3, Yr9, Yr10, Yr15, Yr26, and Yr CH42 on chromosome 1B. Allelic tests indicated that Yr MY41 is likely a new allele at Yr26 locus.

关 键 词：Triticum aestivum L. yellow rust simple sequence repeat（SSR） marker sequence tagged site（STS） marker 

分 类 号：S512.1[农业科学—作物学] S511.03