激素对白三叶愈伤组织形成的影响及体细胞胚的组织学观察  

Effects of Hormone on Callus Induction of White Clover and Histological Observation of Somatic Embryogenesis

作　　者：杨丽[1,2] 张学云[1] 王瑜[1] 袁庆华[1] 

机构地区：[1]中国农业科学院北京畜牧兽医研究所,北京100193 [2]兰州大学草地农业科技学院,甘肃兰州730000

出　　处：《中国草地学报》2015年第1期7-14,共8页Chinese Journal of Grassland

基　　金：国家"十二五"科技支撑项目(2011BAD17B01);国家牧草产业体系(岗位专家)项目资助

摘　　要：以白三叶3个品种瑞文德、铺地和海法的子叶和下胚轴为外植体,采用正交设计试验研究2,4-D、6-BA和KT三种生长调节剂的不同浓度水平对白三叶愈伤组织诱导及继代的影响,筛选最佳激素组合并进行体细胞胚的组织学观察。结果表明:同一品种同一时期不同外植体愈伤诱导的最佳激素组合不相同,不同品种间也不一致;瑞文德子叶胚性愈伤诱导的最佳培养基为改良SH+2,4-D 2mg/L+6-BA 0.35mg/L+KT 0.5mg/L,下胚轴为改良SH+2,4-D 2mg/L+6-BA 0.2mg/L+KT 0.35mg/L;铺地子叶胚性愈伤诱导的最佳培养基为改良SH+2,4-D 2mg/L+6-BA 0.5mg/L+KT 0.5mg/L,下胚轴为改良SH+2,4-D 5mg/L+6-BA 0.35mg/L+KT 0.5mg/L;海法子叶胚性愈伤诱导的最佳培养基为改良SH+2,4-D 2mg/L+6-BA 0.2mg/L+KT 0.35mg/L,下胚轴为改良SH+2,4-D5mg/L+6-BA 0.5mg/L+KT 0.5mg/L。组织学观察表明,体细胞胚的发育经历球形胚、心形胚、鱼雷胚及子叶胚四个阶段后形成一个完整的体细胞胚。The cotyledon and hypocotyl of three varieties of white clover were used as the explants and the effects of different levels of three plant growth regulator 2,4-D、6-BA and KT on white clover callus induction and subculture were studied with orthogonal design,screening the best hormone combinations,and histological observation of somatic embryogenesis was done.The results showed that the best hormone combinations differed with different explants of the same species at the same time,and not consistent between different varieties.The optimum medium for cotyledon of Rivendel varieties seeds was modified SH＋2,4-D 2mg/L＋6-BA 0.35mg/L＋KT 0.5mg/L,for hypocotyl was modified SH＋2,4-D 2mg/L＋6-BA 0.2mg/L＋KT 0.35mg/L;the optimum medium for cotyledon of Prop varieties seeds was modified SH＋2,4-D 2mg/L＋6-BA 0.5mg/L＋KT 0.5mg/L,for hypocotyl was modified SH＋2,4-D 5mg/L＋6-BA 0.35mg/L＋KT 0.5mg/L;the optimum medium for cotyledon of Haifa varieties seeds was modified SH＋2,4-D 2mg/L＋6-BA 0.2mg/L＋KT 0.35mg/L,for hypocotyl was modified SH＋2,4-D 5mg/L＋6-BA 0.5mg/L＋KT 0.5mg/L;The somatic embryos developed through four stages：Globular embryo、Heart-shaped embryo、Torpedo embryo and Cotyledon embryo.

关 键 词：白三叶 正交设计 体细胞胚 生长激素 

分 类 号：Q943.1[生物学—植物学]