四川野生斑茅居群遗传结构的SRAP分析  

SRAP Analysis of Genetic Structure of Wide Erianthus arundinaceum Populations in Sichuan China

作　　者：张瑜[1] 鄢家俊[2] 白史且[1,2] 彭燕[1] 梁绪振[2] 张建波 张劲[2] 胡超 

机构地区：[1]四川农业大学草业科学系,四川雅安625014 [2]四川省草原科学研究院,四川成都611731 [3]贵州省草业研究所,贵州贵阳550006 [4]重庆市垫江实验中学,重庆408300

出　　处：《中国草地学报》2015年第1期15-21,共7页Chinese Journal of Grassland

基　　金：国家高技术研究发展计划("863计划")项目"甘蔗属等新型能源草种质资源挖掘与评价技术研究"(2012AA101801-01)

摘　　要：采用SRAP标记分析了12个源于四川境内长江主要支流(岷江、青衣江和沱江)流域野生斑茅居群的遗传多样性和遗传结构,结果表明:18对SRAP引物共扩增205条带,其中多态性条带153条,占总条带数的74.63%,平均8.5条;研究区域斑茅具有丰富的遗传多样性,遗传多样性指标水平较高(PPB=74.63%、h=0.2278、I=0.3454);研究区域内斑茅居群具有一定程度的遗传分化(Gst=0.2129),居群内部的遗传变异大于居群间的遗传变异;基因流估计值(Nm)为1.8481;按Nei’s遗传距离将12个斑茅居群分为四类,来自同一流域的材料优先聚为一类。通过对斑茅遗传多样性、遗传结构及聚类结果的分析,推测斑茅是一种异花授粉的广布种,说明河流影响斑茅居群间基因的交流。In this experiment,the genetic diversity and genetic structure of 12 wide Erianthus arundinaceum populations collected from tributary of Yangtze River（Minjiang River,Qingyijiang River and Tuojiang River）in Sichuan province in China were investigated by using Sequence-related amplified polymorphism（SRAP）markers.The results showed that：（1）205bands were amplified with 18 pairs of primers,153 bands were polymorphic（PPB=74.63%）,the average polymorphic bands amplified with each primer pair was 8.5.（2）E.arundinaceumin Sichuan province showed high genetic diversity,and genetic diversity indexes showed higher level（PPB=74.63%,h=0.2278,I=0.3454）.（3）Gst（0.2129）showed that most of the genetic variability resided among E.arundinaceumpopulations,and the variability within populations was greater than that among populations,gene flow（Nm）was 1.8481.（4）12 E.arundinaceum populations were clustered into 4groups by Nei＇s genetic distance.Cluster analysis showed that the accessions come from same river would be clustered into one group firstly.Based on the genetic diversity,the genetic structure and cluster analysis of E.arundinaceum,it was suggested that E.arundinaceumis a widespread and cross-pollination species,the river affected the gene flow among E.arundinaceum populations.

关 键 词：斑茅 SRAP 遗传多样性 遗传结构 聚类分析 

分 类 号：S543.9[农业科学—作物学]