下丘脑腹内侧核损毁对大鼠nesfatin-1/NUCB2表达的影响  

The Effects of Hypothalamic Ventromedial Nucleus Lesion on Nesfatin-1/NUCB2 Expression in Rats

作　　者：张婵[1,2] 王巧玲[1] 逄明杰[3] 祝海[3] 郭菲菲[1] 孙向荣[1] 公衍玲[4] 徐珞[1] 

机构地区：[1]青岛大学医学院病理生理学教研室,山东青岛266021 [2]菏泽医学专科学校,山东菏泽274031 [3]青岛市立医院,山东青岛266011 [4]青岛科技大学化工学院,山东青岛266042

出　　处：《现代生物医学进展》2015年第5期818-821,共4页Progress in Modern Biomedicine

基　　金：国家自然科学基金项目(31071014;81100260;81270460;81300281);青岛市科技局项目(13-1-4-170-jch;11-2-3-3-(2)-nsh)

摘　　要：目的:观察下丘脑腹内侧核(VMH)损毁对大鼠脂肪组织nesfatin-1/NUCB2表达的影响及其机制。方法:电损毁VMH,观察大鼠体重和脂肪组织变化,采用Western blot检测脂肪组织中nesfatin-1/NUCB2表达改变。腹腔注射6-羟多巴胺(50 mg/kg)以阻断交感神经;持续外周注射卡巴胆碱(180μg/kg)用以模拟VMH损毁,观察其对大鼠皮下脂肪nesfatin-1/NUCB2表达的影响。结果:与对照组和假手术组比较,VMH损毁后大鼠体重明显增加(P<0.05),皮下脂肪(P<0.05)和肠系膜脂肪(P<0.05)也显著增多;Western blot分析结果显示,nesfatin-1/NUCB2在胰腺和肝脏中表达较多,但皮下、肠系膜脂肪和肩胛间棕色脂肪组织(i BAT)中表达较少,骨骼肌(腓肠肌)中鲜有表达;与对照组和假手术组比较,VMH损毁组大鼠nesfatin-1/NUCB2在肝脏、胰腺、骨骼肌和i BAT中表达无显著差异(P>0.05),皮下脂肪(P<0.05)和肠系膜脂肪(P<0.05)nesfatin-1/NUCB2表达显著增多与对照组相比,6-羟多巴胺组nesfatin-1/NUCB2表达显著升高(t=3.43,P<0.05),而卡巴胆碱组nesfatin-1/NUCB2表达无显著差异(t=0.37,P=0.72)。结论:VMH损毁后大鼠脂肪组织nesfatin-1/NUCB2表达改变可能通过抑制交感神经活动介导。Objective： To observe the effects of ventromedial hypothalamus nuclear lesion on nesfatin-1/NUCB2 expression of white adipose tissue in rats, and to explore its underlying mechanism. Methods： The hypothalamic ventromedial nucleus （VMH） of rats was damaged by electrical stimulation. The changes of weight and adipose tissues of rats were observed. And the expression of nes- fatin-1/NUCB2 in adipose tissues was detected by Western blot analysis. Intraperitoneal injection of 6-hydroxy dopamine （50 mg/kg） was used to block the sympathetic nerve; continuous peripheral injection of kappa choline （180 μg/kg） was used to simulate VMH damaged to observe the nesfatin-1/NUCB2 expression in subcutaneous fat of rats. Results： Compared with the control group and sham-operated group, the weight （P〈0.05）, subcutaneous fat （P〈0.05） and mesenteric fat （P〈0.05） of VMH-lesioncd rats increased significantly. The re- sults of Western blot analysis showed that nesfatin-1/NUCB2 was mainly expressed in the pancreas and liver, less in subcutaneous, mesenteric fat and interscapular brown adipose tissues （iBAT）, little in skeletal muscle （calf）. Compared with the control group and sham-operated group, the expression of nesfatin-1/NUCB2 in liver, pancreas, skeletal muscles and iBAT in VMH-lesioned group had no significant differences, but in subcutaneous fat （P〈0.05） and mesenteric fat （P〈0.05） it significantly increased. Compared with that in the control group, the expression of nesfatin-1/NUCB2 in 6-hydroxy dopamine group increased significantly （t=3.43, P〈0.05）, but was reduced in carbachol group （t=0.37, P=0.72）. Conclusions： These results show that after VMH lesion, the increased expression of nes- fatin-1/NUCB2 in white adipose tissues may be mediated via inhibition of the sympathetic nerve activity.

关 键 词：Nesfatin-1/NUCB2 下丘脑腹内侧核 脂肪组织 自主神经系统 

分 类 号：Q95-3[生物学—动物学] R589[医药卫生—内分泌]