P-cadherin和HSP27蛋白在胃癌组织的表达及临床意义  

Expression and clinical significance of P-cadherin and HSP27 proteins in gastric carcinoma

作　　者：胡风兰[1] 何秋瑜[1] 李祥佩 刘清华[1] 刘莹[1] 朱祖安[2] 

机构地区：[1]徐州医学院病理教研室,江苏徐州221000 [2]徐州医学院附属医院消化内科,江苏徐州221000

出　　处：《南京医科大学学报（自然科学版）》2015年第1期26-30,共5页Journal of Nanjing Medical University(Natural Sciences)

基　　金：江苏省卫生厅指导性科研项目(Z201016)

摘　　要：目的:探讨P钙黏蛋白(P-cadherin)与热休克蛋白27(HSP27)在胃癌组织中的表达及其与临床病理因素之间的关系,进而探讨其作为预后指标的意义。方法:建立组织芯片,通过免疫组化SP法检测75例无术前放化疗史的胃癌手术标本和75例正常胃组织中P-cadherin、HSP27的表达情况,并对随访患者进行生存分析。结果 :在胃癌组织及正常胃组织中,Pcadherin、HSP27的表达率分别为40.0%、82.7%(P<0.001)和64.0%、30.7%(P<0.001)。P-cadherin低表达和HSP27高表达与临床分期、分化程度和淋巴结转移相关(P<0.05),HSP27高表达还与肿瘤大小相关(P<0.05)。Pearson相关分析显示,P-cadherin与HSP27在胃癌中的表达呈负相关(P<0.05)。单因素Kaplan-Meier回归分析显示P-cadherin和HSP27与预后相关(P<0.05)。Cox单因素回归分析显示P-cadherin、HSP27、临床分期、肿瘤大小、有无淋巴结转移与患者预后相关(P<0.05);Cox多因素回归模型分析显示HSP27、临床分期与患者预后相关(P<0.05)。结论:在胃癌中,P-cadherin低表达、HSP27高表达与胃癌临床病理特征和生物学行为密切相关。P-cadherin与HSP27的表达在胃癌组织中呈负相关。P-cadherin和HSP27可作为判断患者预后的候选指标。Objective：This study investigated the expression and significance of both P-cadherin and HSP27 proteins in gastric carcinoma tissues as well as the relationships among the clinical pathologic factors, and discussed the meaning of being prognostic indicators. Methods： High throughput technology of tissue microarray and immunohistochemical SP method was employed to investigate the expression of P-cadherin and HSP27 proteins in 75 gastric carcinoma specimens from patients without chemo-radiation therapy and 75 non-tumor gastric tissues. Survival analysis was carried out on the follow-up of patients. Results： In gastric cancer tissue and normal gastric tissue, the expression rates of P-cadherin, HSP27 were detected to be 40.0% and 82.7%, 64.0% and 30.7%, respectively （both P 〈 0.001）. High expression of P-cadherin and low expression of HSP27 were related to clinical stage, differentiation degree and lymph node metastasis （P 〈 0.05）, the high expression of HSP27 was also correlated with tumor size （P 〈 0.05）. According to the results of Pearson correlation analysis, the expression rates of P-cadhefin and HSP27 in gastric cancer were negatively correlated to each other（P 〈 0.05）. Kaplan-Meier analyses showed that P-cadherin and I-ISP27 were significantly associated with the prognosis （P 〈 0.05）. The results of univariate analysis of Cox showed that P-cadherin, HSP27, clinical stage, tumor size and lymph node metastasis were significantly correlated with the prognosis （P 〈 0.05）; The multivariate analysis of Cox model indicated that HSP27 and clinical stage were significantly correlated with the prognosis （P 〈 0.05）. Conclusion： The low expression of P-cadherin in gastric carcinoma and high expression of HSP27, which was closely related with the clinical pathological features and biological behaviors of gastric cancer. Expression of P-cadherin and HSP27 were negatively correlated in gastric carcinoma. P-cadherin and HSP27 could be used as the candidate index to judge the

关 键 词：组织芯片 P钙黏素 热休克蛋白27 

分 类 号：R735.2[医药卫生—肿瘤]