反相液相色谱下淀粉2-苯甲酸酯-3-(3,5-二甲基苯基氨基甲酸酯)-6-((S)-1-苯基乙基氨基甲酸酯)手性固定相手性识别能力的研究  

Evaluation of Amylose 2-Benzoate-3-( 3,5-dimethylphenylcarbamate)-6-(( S)-1-phenylethylcarbamate) under Reversed-phase Conditions

作　　者：徐红月 唐守万[2] 潘富友[2] 高建荣[1] 

机构地区：[1]浙江工业大学化学工程学院,杭州310014 [2]台州学院医药化工学院,台州318000

出　　处：《科学技术与工程》2015年第3期5-10,共6页Science Technology and Engineering

基　　金：国家自然科学基金青年科学基金项目(20905057)资助

摘　　要：合成了基于淀粉2-苯甲酸酯-3-(3,5-二甲基苯基氨基甲酸酯)-6-((S)-1-苯基乙基氨基甲酸酯)的手性固定相,对其进行了表征,考察了其在反相色谱条件下的手性识别能力。对中性手性化合物的分离表明:流动相中有机添加剂的含量及性质均对对映体的保留和分离产生影响。与以乙腈为流动相添加剂相比,流动相添加剂为甲醇时,手性固定相显示出更高的手性识别能力;同时对映体在手性固定相上的保留更强。相比于正相色谱,所制备的固定相在反相色谱下通常显示出更低的手性识别能力;但对映体1在反相色谱条件下得到了更好的分离,其α值普遍高于其在正相色谱条件下的α值。同时,还研究了酸性、碱性手性化合物的分离;但在实验条件下他们均没有得到拆分。A chiral stationary phase（ CSP） based on amylose 2-benzoate-3-（ 3,5-dimethylphenylcarbamate）-6-（（ S）-1-phenylethylcarbamate） was prepared,characterized,and evaluated for HPLC enantioseparations under reversed-phase conditions.For the separation of neutral chiral compounds,investigations indicated that the retention and resolution of the racemates were influenced by the nature and the content of the organic additive in the mobile phase.Compared with using acetonitrile as the mobile phase additive,when methanol was used,the CSP exhibited higher chiral recognition and stronger retention was attained.Compared with the resolution data under normal phase conditions,the CSP generally exhibited lower chiral recognition under reversed-phase conditions,while racemate 1was generally better resolved with a higher α value.Also,the resolution of acidic and basic chiral compounds were carried out;however,none of them were separated.

关 键 词：手性固定相 手性识别 反相色谱 多糖衍生物 

分 类 号：O636.12[理学—高分子化学]