二倍体马铃薯在盐胁迫下试管薯的诱导及生理特性的反应  

Tuber Trait and Physiological Index of Microtuber of Diploid Potato Under Salt Stress

作　　者：张景云[1,2] 白雅梅[1] 缪南生[2] 万新建[2] 李文霞[1] 吕文河[1] 

机构地区：[1]东北农业大学,黑龙江哈尔滨150030 [2]江西省农业科学院蔬菜花卉研究所,江西南昌330200

出　　处：《核农学报》2015年第1期139-145,共7页Journal of Nuclear Agricultural Sciences

基　　金："十一五"国家863项目(2006AA100107)

摘　　要：本研究旨在研究育体条件下盐脉迫对二倍体马铃薯富利亚和窄刀薯杂种(PHU-STN)无性系间的差异。以耐盐、中度耐盐、感盐的二倍体马铃薯富利亚和窄刀薯杂种无性系各5份为试验材料,用0(对照)、30 mmol·L-1的Na Cl进行胁迫,通过试管薯直径、个数、最大薯薯重、产量及生理指标的测定,观察不同耐盐性马铃薯试管薯的诱导情况及生理特性的变化。结果表明,盐胁迫后,试管薯直径、个数、最大薯薯重、产量均降低,SOD活性升高,POD活性降低,相对电导率和丙二醛含量升高,且二倍体马铃薯组间各性状差异均达极显著水平。因此,试管薯块茎性状以及生理特性SOD、POD活性、电导率和丙二醛含量可以作为二倍体马铃薯耐盐性鉴定的指标。The purpose of this research was to study the difference between hybrid clones（ PHU-STN） of solanum phureja（ PHU） and S. stenotomum（ STN） in vitro under salt stress. Five clones of each of salt tolerant,medium salt tolerant,and salt susceptible groups selected from a Solanum phureja-S. stenotomum hybrid population were evaluated for their diameter,number,the largest tuber weight,yield,and physiological indexes of in vitro microtuber to understand the change in tuber trait and physiological index of microtuber under salt stresses of 0（ control） and 30 mmol·L- 1Na Cl. The diameter,number,the largest tuber weight,yield,and peroxidase（ POD） activity of microtuber were decreased under salt stress,while superoxide dismutase（ SOD） activity,electric conductivity,and malondialdehyde（ MDA） increased. Furthermore,the differences among the three groups for these tuber traits and physiological indexes were highly significant. Therefore, tuber traits and physiological indexes（ SOD activity, POD activity, electric conductivity,and MDA content） of in vitro microtuber could be used as indicators for identification of salt tolerant diploid potatoes.

关 键 词：盐胁迫 试管薯 块茎性状 生理特性 

分 类 号：S532[农业科学—作物学] Q945.78[生物学—植物学]