锰胁迫对大豆氮素积累的影响  

Effect of Manganese Stress on Nitrogen Accumulation of Soybean

作　　者：金喜军[1] 屈春媛 赵云娜[1] 栗文霞 迟超 张玉先[1] 王景伟[1] 

机构地区：[1]黑龙江八一农垦大学农学院,黑龙江大庆163319 [2]黑龙江省闫家岗农场,黑龙江哈尔滨150078

出　　处：《核农学报》2015年第1期146-152,共7页Journal of Nuclear Agricultural Sciences

基　　金：国家自然科学基金项目(204134008);黑龙江八一农垦大学博士启动基金(XYB2013-03);黑龙江八一农垦大学作物学科学技术骨干科研启动金项目(ZWXQDJ-4)

摘　　要：采用15N示踪技术与沙培相结合的方法,以最适锰浓度为对照,设置锰缺乏(分别为对照浓度的1/25和1/5作为重度和轻度锰缺乏处理)和锰过量(分别为对照浓度的5倍和25倍作为轻度和重度锰过量处理)4个锰胁迫处理,研究了锰胁迫对大豆氮素积累的影响。结果表明:与对照相比,锰胁迫并未明显影响生育前期大豆植株氮素积累,但对中后期氮素积累起显著抑制作用(P<0.01),并且抑制作用随胁迫程度的加深而加重;锰胁迫对苗期(V4)肥料氮的积累无影响,但对其他生育时期肥料氮的积累均表现出不同程度的抑制作用;在整个生育期间,锰胁迫显著抑制了根瘤固氮的积累,大幅度减少了鼓粒期间根瘤固氮的积累;锰胁迫不仅显著减少了成熟期(R8)大豆营养器官和子粒中肥料氮、根瘤固氮、总氮的积累,还在一定程度上增加了营养器官中肥料氮、根瘤固氮、总氮的积累比例,子粒则相应减小。综合分析表明,锰胁迫对大豆肥料氮、根瘤固氮、以及总氮的积累均有抑制作用,抑制效果在大豆生育中后期表现明显,并且锰胁迫改变了成熟期大豆营养器官和子粒中肥料氮、根瘤固氮和总氮的积累比例。本试验所得结果丰富了锰胁迫与大豆氮素营养的相关研究内容,并为进一步的机理研究奠定了基础。In this experiment,soybean was cultivated with sand culture method combined with15 N tracer technique.Optimal Mn concentration was used as control,and 4 manganese stress treatments including 2 manganese deficiency treatments（ including serious stress and light stress which were 1 /25 and 1 /5 of optimal Mn concentration respectively）and 2 excess stress treatments（ including serious stress and light stress which were 5 and 5 times of optimal Mn concentration respectively） were set to study the effect of manganese stress on soybean nitrogen accumulation. The results were shown that： compared to control,manganese stress had no significant effect on nitrogen accumulation of soybean plant at earlier growing stage,but significantly inhibited that at middle and late growth stage（ P 0. 01）,and the inhibition degree increase with an increased in stress degree. Manganese stress had no effect on Fertilizer-N accumulation at seedling period（ V4）,but significantly inhibited that at other growth stage. In the all stages,manganese stress significantly inhibited Nodulation-N accumulation,and greatly reduced that during the seed filling period.Manganese stress not only reduced the accumulation of Fertilizer-N and Nodulation-N in vegetative organs and seed,but also increased the ratio between Fertilizer-N and Nodulation-N in vegetative organs and seed and Fertilizer-N and Nodulation-N in the whole plant at mature stage（ R8）,that of seed was exactly the opposite. The result indicated that：manganese stress inhibited the Fertilizer-N,Nodulation-N,and total N accumulation in soybean plant,and this inhibition effect exhibited significantly at middle and late growth stage,manganese stress also changed the the ratio between Fertilizer-N,Nodulation-N,total N in vegetative organs and seed and Fertilizer-N,Nodulation-N,total N in the whole plant at mature stage（ R8）. Results of this coticle has enriched the research content between manganese stress and nitrogen nutrition of soybean,and provides basis for revealin

关 键 词：大豆 锰胁迫 氮素积累 肥料氮 根瘤固氮 

分 类 号：S565.1[农业科学—作物学] Q945.78[生物学—植物学]