Small RNA existed in commercial reverse transcriptase： primary evidence of functional small RNAs  

作　　者：Jie Xu Xi Chen Donghai Li Qun Chen Zhen Zhou Dongxia Hou Jin Wang Qipeng Zhang Ke Zen Chen-Yu Zhang 

机构地区：[1]Jiangsu Engineering Research Center for microRNA Biology and Biotechnology, State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing 210093, China

出　　处：《Protein & Cell》2015年第1期1-5,共5页蛋白质与细胞（英文版）

基　　金：We would like to thank Mr. Chao Gao for his technical support. This work was supported by grants from the National Natural Science Foundation of China （Grant Nos. 31100777, 30988003 and 90608010） and the Natural Science Foundation of Jiangsu Province （BK2011013）. Jie Xu, Xi Chert, Donghai Li, Qun Chen, Zhen Zhou, Dongxia Hou, Jin Wang, Qipeng Zhang, Ke Zen and Chen-Yu Zhang declare that they have no conflict of interest. All procedures followed were in accordance with the ethical standards of the responsible committee on human experimentation （institutional and national） and with the Helsinki Declaration of 1975, as revised in 2000. Informed consent was obtained from all patients for being included in the study. All institutional and national guidelines for the care and use of laboratory animals were followed.

摘　　要：SMALL RNA IN REVERSE TRANSCRIPTASE： TOO SMALL TO BE FOUND Significant background in no-template or no-primer RT-PCR is frequently noted. However, the underlying mechanism is still unclear. In this issue, Jie Xu and colleagues reveal that the com- mercial reverse transcriptases contain small RNAs. In AMV reverse transcriptase, they detected microRNAs derived from the virus host chick or other birds. This surprising finding at least partially explains the high background in some AMV reverse transcriptases based RT-PCR.SMALL RNA IN REVERSE TRANSCRIPTASE： TOO SMALL TO BE FOUND Significant background in no-template or no-primer RT-PCR is frequently noted. However, the underlying mechanism is still unclear. In this issue, Jie Xu and colleagues reveal that the com- mercial reverse transcriptases contain small RNAs. In AMV reverse transcriptase, they detected microRNAs derived from the virus host chick or other birds. This surprising finding at least partially explains the high background in some AMV reverse transcriptases based RT-PCR.

分 类 号：Q55[生物学—生物化学] S511[农业科学—作物学]