内质网应激和氧化应激在纳米氧化铈所致肺损伤中的作用  

Effects of Endoplasmic Reticulum Stress and Oxidative Stress on Pulmonary Injury Induced by Nano CeO_2 in Rats

作　　者：杨静[1] 吴刚[1] 李园园 吕丽萍[3] 刘扬[1] 庞一强[4] 

机构地区：[1]包头医学院基础医学与法医学院,内蒙古包头014040 [2]鄂尔多斯市卫生学校,内蒙古鄂尔多斯017000 [3]包头医学院研究生学院,内蒙古包头014040 [4]包头市第四医院神经外科,内蒙古包头014030

出　　处：《动物医学进展》2015年第3期73-75,共3页Progress In Veterinary Medicine

基　　金：国家自然科学基金项目(81160341);内蒙古自治区高等学校科学研究项目(NJZY13238)

摘　　要：观察不同剂量纳米氧化铈颗粒染毒后对大鼠血清超氧化物歧化酶(SOD)活力和丙二醛(MDA)含量的影响及大鼠肺组织GRP78蛋白表达情况,探讨其导致肺损伤的作用机制。将粒径为35nm±3nm的纳米氧化铈颗粒悬液以不同剂量(100mg/kg、400mg/kg)给大鼠气管滴注,染毒28d后,测定大鼠血清中SOD活力、MDA含量及肺组织GRP78蛋白表达情况。与对照组相比,高低剂量纳米氧化铈染毒组大鼠血清中SOD活力及MDA含量均无明显改变(P>0.05),GRP78蛋白表达均显著增高(P<0.05)。纳米氧化铈染毒大鼠所致肺损伤与内质网应激有关,与氧化应激的关系有待进一步研究。To investigate the effects on the levels of serum SOD and MDA in rats and the expression of GRP78 in lungs of rats treated with different doses nano CeO2 ,and study the mechanism of pulmonary inj ury induced by nano CeO2 ,the suspension of nano CeO2 （35 nm±3 nm）of different doses （400 mg/kg,100 mg/kg）were administrated via intratracheal instillation in rats.After 28 days,the levels of serum SOD and MDA and the expression of GRP78 were observed.In comparison with the control,the levels of serum SOD and MDA treated with different doses of nano CeO2 had no significant change（P〉0.05）,the expression of GRP78 in all groups increased significantly（P〈0.05）.Pulmo-nary inj ury induced by nano CeO2 in rats was related to endoplasmic reticulum stress,and the relationship between pul-monary inj ury and oxidative stress should be further researched.

关 键 词：纳米氧化铈 肺损伤 内质网应激 氧化应激 

分 类 号：S852.33[农业科学—基础兽医学]