酪氨酸磷酸酶基因ptpn11对乳腺肿瘤生长的影响  

Effect of ptpn11 on Breast Tumorigenesis in Animal Model

作　　者：田莹普 张智英[1] 

机构地区：[1]西北农林科技大学动物科技学院基因组实验室,陕西杨凌712100

出　　处：《动物医学进展》2015年第3期83-87,共5页Progress In Veterinary Medicine

摘　　要：乳腺癌是威胁妇女健康和生命的头号恶性肿瘤,但其发生发展的机制及诊疗仍然没有长足的进展。蛋白酪氨酸磷酸酶11(PTPN11)是一个易于突变的基因,参与乳腺肿瘤的发生,但目前关于PTPN11调控乳腺癌的体内证据仍然缺乏。为了明确PTPN11在体内对乳腺癌发生发展的调控作用,利用大鼠乳腺肿瘤的动物模型,用PTPN11蛋白酶活性抑制剂Phps1处理,然后观察乳腺肿瘤发生的情况。结果发现Phps1处理后可延长DMBA诱导的大鼠乳腺肿瘤的潜伏期,延迟肿瘤的发生,并显著降低了DMBA诱发肿瘤的比率(P<0.05),但对肿瘤生长的抑制作用不显著。证明了PTPN11参与调控体内乳腺肿瘤的发生,为理解和诊治乳腺癌提供了一个可能新的靶点。Breast cancer is one of the major disease threats to women＇s health and life,and has become the top one in women＇s malignant tumor list.The human gene ptpn1 1 （coded protein tyrosine phosphatase ）is easy to be mutated,and involved in the occurrence of breast tumor.But the evidences of the regulation in vivo of PTPN1 1 on breast cancer are still lacking.To investigate the role of PTPN1 1 in the development of breast cancer invivo,we treated the rat with DMBA-induced breast tumor by the activity inhibitor （Phps1） of PTPN1 1 and observed the tumor in rats.We found that Phps1 delayed the occurrence of DMBA-induced breast tumor in rats.The proportion of DMBA-induced tumor was significantly reduced （P〈0.05）.The number of tumors in Phps1-treated rats had a reduction of 7%-10%.But the tumor growth was not blocked.These results are preliminary evidence that PTPN1 1 involved in regulating the occurrence of breast tumor invivo.

关 键 词：蛋白酪氨酸磷酸酶11(PTPN11) 乳腺肿瘤动物模型 二甲基苯蒽(DMBA) PTPN11蛋白酶活性抑制剂 

分 类 号：S852.31[农业科学—基础兽医学] S857.4[农业科学—兽医学]