新乡市某猪场猪瘟病毒的鉴别检测  

Identifiction and Detection of Classical Swine Fever Virus in A Pig Farm of Xinxiang

作　　者：孔令芸 陈玲丽[1] 李冲[1] 孙相和 李鹏[2] 王选年[2] 银梅[1] 

机构地区：[1]河南科技学院动物科学学院,河南新乡453003 [2]新乡学院生命科学与技术系,河南新乡453003

出　　处：《动物医学进展》2015年第3期120-123,共4页Progress In Veterinary Medicine

基　　金：国家自然科学基金项目(31201877)

摘　　要：新乡市某猪场发生疑似猪瘟病例,为了鉴别其为疫苗毒还是野毒,采用细胞免疫化学方法和RT-PCR,并对其NS5B基因进行序列测定,序列比对并构建进化树,结果表明,猪只为猪瘟野毒感染,且分离的猪瘟病毒与石门(Shimen)株在基因序列上未发生大的变异。The suspicious cases of classical swine fever occurred in a pig farm of Xinxiang.The virus was i-dentified by cell immunochemistry method and RT-PCR.The results of the sequence alignment and phylo-genetic tree of NS5B gene showed that the pigs were infected with classical swine fever virus field strain, and the gene sequence variation of this isolate was not obvious.

关 键 词：猪瘟病毒 免疫细胞化学 反转录-聚合酶链反应 序列分析 

分 类 号：S852.651[农业科学—基础兽医学] S858.28[农业科学—兽医学]