温度响应的主客体超分子人工硒酶构建及催化机制研究  

Construction of a Supramolecular Host-Guest Artificial Glutathione Peroxidase with Temperature Responsive Activity and the Investigation of Responsive Catalytic Mechanism

作　　者：焦淑菲[1] 尹艳镇[1] 史忠丰[1] 胡晓熙[1] 王学谦[1] 张丽[1] 杨兰[1] 

机构地区：[1]钦州学院化学化工学院,钦州535000

出　　处：《材料导报》2015年第2期38-42,66,共6页Materials Reports

基　　金：广西自然科学基金青年基金项目(2013GXNSFBA019043);广西高校科学技术研究项目(2013YB254);国家自然科学基金青年科学基金项目(51303088;51203082);广西壮族自治区大学生创新创业计划项目(201411607033)

摘　　要：基于环糊精/金刚烷主客体作用制备了催化活力具有温度响应的超分子仿生硒酶（PNIPAM-CD-Te）。研究表明,PNIPAM-CD-Te的催化活力在25-45℃温度区间内具有典型的温度响应特性,37℃时具有最高催化活力（υ0=4.97mmol·L^-1·min^-1）;同时,PNIPAM-CD-Te还展现出典型的酶催化饱和动力学行为。此外,研究表明,PNIPAM-CD-Te聚合物骨架在温度响应过程中会发生自组装结构的变化,这一变化对调控PNIPAM-CD-Te的底物结合能力具有重要作用,是导致PNIPAM-CD-Te催化活力具有温度响应特性的根本原因。A supramolecular artificial glutathione peroxidase（PNIPAM-CD-Te）with temperature responsive activity was constructed based on the host-guest supramoleuclar interaction of cyclodextrin/adamantane system.It was proved that PNIPAM-CD-Te displayed a noticeable temperature responsive catalytic activity from 25℃to 45℃.The highest catalytic rate was obtained at 37℃（υ0=4.97mmol·L^-1·min^-1）.And PNIPAM-CD-Te exhibited the typical saturation kinetic behavior as a real enzyme catalyst.Additionally,it was found that the self-assembled structure of PNIPAM-CD-Te was changed during the temperature responsive process.And such change played a significant role in altering the substrates binding ability of PNIPAM-CD-Te,which was the reason for the temperature responsive catalytic behavior.

关 键 词：超分子 主客体 仿生硒酶 温度响应 催化机制 

分 类 号：TB324[一般工业技术—材料科学与工程]