丙烯酰胺对小鼠成体神经发生及GSK3β表达的影响  

Influence of acrylamide on adult neurogenesis and expression of GSK3β in mouse

作　　者：李凯凯[1] 谢炅芳 王久涛[1] 宋玲珍[1] 张伟[1] 陈树林[1] 赵善廷[1] 

机构地区：[1]西北农林科技大学动物医学院,杨陵712100

出　　处：《中国免疫学杂志》2015年第2期163-168,共6页Chinese Journal of Immunology

摘　　要：目的:探讨和研究丙烯酰胺对成年小鼠海马成体神经发生及GSK3β表达的影响。方法:雄性成年昆明小鼠分为丙烯酰胺处理组和生理盐水对照组,通过腹腔注射(i.p.)进行丙烯酰胺染毒,用Brd U标记和免疫组织化学方法研究齿状回神经干细胞的增殖,Brd U/Neu N/GFAP三标的方法研究齿状回神经干细胞的存活和分化,Neuro-2a细胞中观察GSK3β表达与分布,用Western blot方法检测小鼠海马中GSK3β的表达。结果:丙烯酰胺处理组小鼠齿状回亚颗粒细胞层(SGZ)中的Brd U阳性细胞数量显著低于对照组,并且新生细胞向神经元分化的数量显著降低。丙烯酰胺处理小鼠SGZ区新生干细胞的存活率显著低于对照小鼠,另外,丙烯酰胺组新生干细胞向胶质细胞分化的比例显著升高。在Neuro-2a细胞中磷酸化的GSK3β表达量极显著升高,但在丙烯酰胺处理组小鼠的海马中磷酸化的GSK3β显著降低。结论:结果表明,丙烯酰胺能显著抑制小鼠齿状回成体神经干细胞增殖,降低新生干细胞的存活率并影响其分化,丙烯酰胺抑制小鼠成体神经发生可能与GSK3β有一定的关系。Objective：To investigate the influence of acrylamide （ACR） on adult neurogenesis and expression of GSK3β in mouse. Methods： Method Adult male Kunming mice were used and divided into two groups：control group and experimental poisoning groups, that were exposured to acrylamide by intraperitoneal injection. Brdu labeling and immunohistochemistry were used to investigate the proliferation of adult neural stem cells in the subgranular zone（SGZ）. BrdU/NeuN/GFAP triple labeling to investigate the survival and differentiation of newly generated cells. Detecting GSK3β expression and distribution in Neuro-2a cells, the expression of GSK3β was examined by using Western blot. Results： Compared with control mice,lower number of BrdU-positive cells and less differentiated into neurons in ACR mice. Less neural stem cells survived, but more glia cells were generated in the subgranular zone of acrylamide mice. Moreover,higher phosphorylated GSK3β （Ser9） were detected in Neuro-2a cells and mouse dentate gyms in ACR mice respectively. Conclusion： These results suggested that acrylamide inhibits neural stem cells proliferation and influences the survival and differentiation of newly generated ceils. Aerylamide inhibits neurogenesis maybe through GSK3β signaling pathway.

关 键 词：丙烯酰胺 成体神经发生 免疫组织化学 GSK3Β 

分 类 号：Q954.48[生物学—动物学]