雪峰虫草对DC-CIK增殖及HepG-2细胞杀伤作用的实验研究  

Effects of Ophiocordyceps xuefengensis on proliferation of DC-CIK cells and activity of killing HepG-2 cells by DC-CIK cells

作　　者：郑兵[1] 谢芳一[1] 蔡国辉 朱如彩[1] 李柯[1] 高守泉[1] 谭电波[1] 郝晓勇 秦裕辉[1] 

机构地区：[1]湖南省中医药研究院中药研究所,长沙410013 [2]长沙厚益深泰医药科技有限公司,长沙410013

出　　处：《中国免疫学杂志》2015年第2期189-192,共4页Chinese Journal of Immunology

基　　金：湖南省科技重大专项资助项目(2014FJ1007);湖南省中医药科研计划项目(201267;201322)资助

摘　　要：目的:研究雪峰虫草对DC-CIK细胞增殖及肝癌Hep G-2细胞杀伤作用。方法:常规分离健康人外周血单个核细胞并诱导生成DC细胞和CIK细胞,将DC细胞与CIK细胞按1∶5共培养7 d后给药组加入不同浓度的雪峰虫草水提取物,第10天观察形态并计数各组DC-CIK细胞;收集培养第10天的DC-CIK细胞作为效应细胞,对数生长期Hep G-2肝癌细胞作为靶细胞,使Hep G-2∶DC-CIK靶效比为1∶5,cck-8法检测DC-CIK对Hep G-2的杀伤率。结果:雪峰虫草水提物组对DC-CIK有显著的促增长作用,其作用的最佳浓度为0.1 mg/ml。雪峰虫草诱导的DC-CIK细胞对肝癌Hep G-2细胞的杀伤作用优于常规方法培养的DC-CIK细胞;常规方法培养的DC-CIK细胞加雪峰虫草与常规方法培养的DC-CIK细胞对肝癌Hep G-2细胞的杀伤作用无显著性差异,雪峰虫草体外直接杀伤肝癌Hep G-2细胞的作用不明显。结论:雪峰虫草通过促进DC-CIK细胞增殖而增强其杀伤肝癌Hep G-2细胞的作用。Objective：To study the effects of Ophof Chinese Materia Medica, Hunan Academy of Chinese Medicine, iocordyceps xuefengensis on proliferation of DC-CIK cells and the activity of killing HepG-2 cells by DC-CIK cells. Methods： Peripheral blood mononuclear cells were routinely isolated and induced into DC and CIK. DC and CIK co-cultured by 1：5 for 7 days, then Ophiocordyceps xuefengensis were added into medicine group , observed the mor- phology of the cells on the tenth day and counted the DC-CIK number of each group. DC-CIK cells acted as effector cells and the HepG- 2 cells as target cells, cck-8 method for the detection of DC-CIK in the killing rate of HepG-2. Results： The Ophiocordyeeps xuefengensis was able to proliferate the DC-CIK dramatically, the optimal concentration was 0. 1 mg/ml. Cultivation of Ophiocordyceps xuefengensis induced DC-CIK cells on HepG-2 cells killing effect was better than that of the routine method of DC-CIK cells; the effection of Ophioeordyeeps xuefengensis killing HepG-2 cells was not obviously. Conclusion： Ophioeordyceps xuefengensis can enhance the anti-tumor activity of DC-CIK mainly by promoting the proliferation of it.

关 键 词：雪峰虫草 树突细胞 杀伤细胞 HEP G-2细胞 

分 类 号：R285.5[医药卫生—中药学]