水通道蛋白2在梗阻性黄疸大鼠肾脏表达变化的实验研究  

Experimental investigates on changes of expression of Aquaporin2(AQP2 ) in renal of rats with obstructive jaundice

作　　者：韩继龙[1] 王勇[1,2] 刘金钢[1,2] 

机构地区：[1]沈阳医学院附属第二医院普外科,沈阳110002 [2]中国医科大学附属盛京医院胆道,疝,减肥外科,沈阳110004

出　　处：《中国免疫学杂志》2015年第2期261-264,共4页Chinese Journal of Immunology

摘　　要：目的:明确水通道蛋白2(AQP2)在梗阻性黄疸大鼠肾脏表达变化及其与尿素氮(BUN)和肌酐(Cr)变化的关系。方法:采用手术结扎胆总管的方法造成实验大鼠梗阻性黄疸模型,于术后3、5、7、10、14 d处死大鼠取血和肾脏标本,检测血浆直接胆红素、尿素氮和肌酐值,光镜下观察肾脏病理形态学,免疫组化方法检测肾脏APQ2表达。结果:实验组3 d时光镜下见肾集合管上皮细胞排列不规则肿胀,5 d时可见细胞水样空泡变性、并且梗阻性黄疸组AQP2表达较对照组明显减少。7 d时见部分细胞脱落、间质有炎性细胞浸润,10和14 d时见局灶上皮细胞坏死、胞浆崩解、间质有大量炎性细胞浸润。水通道蛋白2表达在梗阻5 d后较对照组明显减少(P<0.05),随着梗阻时间延长,其表达减少愈加明显。AQP2表达减少的同时,肾形态学损害在光镜下从肾集合管形态学改变得到证实。梗阻性黄疸组术后10 d Cr开始升高,梗阻14 d后BUN开始升高。结论:AQP2的减少表明肾功能发生损害,AQP2减少在前,CR和BUN增加在后,所以AQP2作为肾功能损害的早期检测指标是很有意义的。Objective： To investigate the change of expression of AQP2 in renal of obstructive jaundice rats , and the relationship between AQP2 and the changing of Cr and BUN. Methods： Legated the common bile duct of rats to form the obstructive jaundice group. Scarified the rats on the 3th ,5th,7th, 10th, 14th day ,took blood samples and the kidney of the rats. Test direct bilirubin BUN and Cr in serum, the renal histopathological changes were observed by optical microscopy. The expression of AQP2 in renal of rats was tested by using radio immunological method. Results： Light microscopic examination of kidney showed that swelling epithelium arranged irregularly in 3rd day ,bleb in the 5th day. The expression of AQP2 in renal of rats with obstructive jaundice were greatly less than sham operation. Mesenchyma inflammatory cells infiltrate in 7th day. Local epithelial necrosis and lots of inflammatory cells infiltrate in the mesenchyma in 10th day and 14th day. The expression of AQP2 in renal collective tubule was decreased on the 5th day compared with sham operation, and the decrease was more as time gone by. The renal function injury can be confirmed though the renal collective tubule change. Cr and BUN in serum began to increase on the 10th day and 14th day. Conclusion： Decrease of AQP2 is earlier than the increase of Cr and BUN, and it can be the early sign of renal function injury.

关 键 词：梗阻性黄疸 水通道蛋白 肾功损害 

分 类 号：R657.43[医药卫生—外科学]