松针对呼吸道合胞病毒感染小鼠肺组织TLR3、TLR4表达的影响  

The Effective of Pine on the Expression of TLR3,TLR4 of Respiratory Syncytial Virus Infection in Mice

作　　者：王杰[1] 张文斌[1] 牟界 雷文汇 李芹[1] 

机构地区：[1]重庆市中医院呼吸内科,重庆400010

出　　处：《世界中医药》2015年第1期86-88,共3页World Chinese Medicine

基　　金：国家中医临床研究基地业务建设科研专项(编号:JDZX2012062)

摘　　要：目的:通过观察松针对呼吸道合胞病毒感染小鼠肺组织Toll样受体3(TLR3)及Toll样受体4(TLR4)蛋白表达的影响,探讨其治疗呼吸道合胞病毒感染的可能免疫调节机制。方法:采用美国癌症研究所(Institute of Cancer Research)培育的ICR小鼠60只随机分为6组:正常对照组、模型组、利巴韦林组、松针高剂量组、中剂量组呼、低剂量组,呼吸道合胞病毒滴鼻后给药,观察肺组织TLR3及TLR4蛋白的表达。结果:松针高剂量组可降低小鼠肺组织TLR3及TLR4蛋白的表达,与模型组比较差异有统计学意义(P<0.05)。结论:松针可抑制呼吸道合胞病毒感染小鼠肺组织中TLR3及TLR4蛋白的表达,可能为其免疫调节作用的机制之一。Objective：To observe the effect of pine in respiratory syncytial virus infection in the expression of TLR3 lung tissue and TLR4 protein in mice,and explore the immune regulation mechanism of the treatment of respiratory syncytial virus infection. Methods：60 ICR mice were randomly divided into 5 groups： normal control group,model group,the antiviral oral liquid group, ribavirin,and pine needles.After intranasal administration of respiratory syncytial virus,observe TLR3 lung tissue and the expres-sion of TLR4 protein.Results：The antiviral oral liquid,ribavirin,pine needles can reduce the TLR3 and the expression of TLR4 protein of lung tissue in mice,compared with model group with significant difference （ P〈0.05） .Conclusion： Pine needles can inhibit the respiratory syncytial virus infection in the lung tissue of mice TLR3 and the expression of TLR4 protein,and may be one of the mechanisms of immune regulation.

关 键 词：松针 呼吸道合胞病毒感染 TLR3 TLR4 

分 类 号：R285.5[医药卫生—中药学]