黄芪含药血清对血管紧张素Ⅱ致内皮细胞凋亡的保护作用  

Protective effects of serum contained huangqi on endothelial cell apoptosis induced by angiotensinⅡ

作　　者：邱宇安[1] 陈火国[2] 李丽红[2] 靳文剑[2] 黄绍烈[3] 

机构地区：[1]江西省肿瘤医院ICU,南昌330029 [2]江西省肿瘤医院内四科,南昌330029 [3]南昌大学第一附属医院心内科,南昌330006

出　　处：《重庆医学》2015年第6期741-742,745,共3页Chongqing medicine

基　　金：江西省卫生厅中医药基金项目(2011A061)

摘　　要：目的观察血管紧张素Ⅱ(AngⅡ)对人脐静脉内皮细胞(HUVECs)的致凋亡效应及黄芪含药血清的保护作用。方法将培养的ECV-304分为以下3组:(1)空白对照组;(2)AngⅡ诱导组,使培养基中AngⅡ终浓度为0、1×10-6、1×10-5、1×10-4 mol/L,与细胞共孵育18h。MTT法检测AngⅡ对HUVECs生长增殖的影响;流式细胞仪检测AngⅡ作用后内皮细胞凋亡率的变化;电子显微镜观察AngⅡ诱导后内皮细胞的超微结构特点;(3)黄芪含药血清干预组,培养基中加入不同浓度黄芪含药血清培养24h后,加入AngⅡ(1×10-4 mol/L)孵育18h,检测内皮细胞凋亡率的变化。结果不同浓度的AngⅡ均能抑制内皮细胞生长增殖。不同浓度的AngⅡ均可显著诱导内皮细胞凋亡。透射电镜下可见AngⅡ诱导后内皮细胞的凋亡形态。黄芪含药血清抑制AngⅡ所诱导的内皮细胞凋亡。结论黄芪含药血清具有内皮保护作用。Objective To study AngⅡ induced apoptosis of HUVECs and to observe the protective effect of serum contained huangqi on endothelial cell.Methods ECV-304 cells were randomly divided into control group,AngⅡ group and huangqi group.In the control group,cells were cultured for 18 h,and the concentration of AngⅡ were 0mol/L,1×10^-6 mol/L,1×10^-5 mol/L and1×10^-4 mol/L.The cell proliferation was measured by MTT assay.Electron microscope was used to observe the change of HUVECs.Ultrastructure of HUVECs induced by AngⅡ was observed by electron microscope.In the huangqi group,serum contained huangqi of different concentration were added into the medium and cultured for 24 h,then AngⅡ of 1×10^-4 mol/L was included and cultured for 18 h,and the apoptosis ratio induced by AngⅡ was measured by flow cytometry.Results AngⅡ of different concentration could all significantly inhibit HUVECs proliferation.AngⅡ of different concentration could all induce endothelial cell apoptosis.HUVECs apoptosis was observed by electron microscope.HUVECs apoptosis induced by AngⅡ could be inhibited by serum contained huangqi.Conclusion Serum contained huangqi could protect endothelial cells.

关 键 词：血管紧张素Ⅱ 内皮细胞 凋亡 黄芪 

分 类 号：R285.5[医药卫生—中药学]