柳树叶酸预处理工艺的优化  

Optimization of Acid Ppretreatment Conditions of Willow Leaves

作　　者：崔茂金[1] 祝勇[1] 周玉[1] 

机构地区：[1]河南科技学院化学化工学院,河南新乡453003

出　　处：《广州化工》2015年第3期57-59,共3页GuangZhou Chemical Industry

基　　金：河南科技学院2011年科研启动经费(210010611)

摘　　要：以柳树叶为底物,通过正交实验和响应曲面法优化了底物的稀HCl预处理工艺。首先通过四因素五水平正交实验研究了预处理温度、HCl浓度、预处理时间和底物浓度对总还原糖(TRS)的影响;然后在正交实验的基础上,通过Box-Behnken设计对柳树叶的预处理工艺进行进一步优化。研究结果表明:影响柳树叶HCl预处理工艺的四个因素的主次顺序为HCl浓度、预处理温度、预处理时间和底物浓度。最佳预处理工艺为HCl浓度0.90%、预处理温度123.62℃、预处理时间53.06 min和底物浓度9.45 g/L,此时总还原糖浓度最大为378.44 mg/g,说明HCl预处理可以较好地提高柳树叶的糖化率。Orthogonal experiment and response surface methodology were used to optimize the pretreatment conditions of hydrochloric acid（ HCl） using willow leaves as substrate. Four- factor- five- level orthogonal experiment was used to study the effect of pretreatment temperature,HCl concentration,pretreatment time and substrate concentration on the total reducing sugar（ TRS）. On the basis of orthogonal experiment,Box- Behnken design was further used to optimize acid pretreatment conditions of willow leaves. The results showed that the primary and secondary order of four factors which effect HCl pretreatment conditions of willow leaves were HCl concentration,pretreatment temperature,pretreatment time and substrate concentration. The optimal HCl pretreatment conditions were HCl concentration of0. 90%,pretreatment temperature of 123. 62 ℃,pretreatment time of 53. 06 min and substrate concentration of 9. 45 g /L. The maximum of total reducing sugar concentration was 378. 44 mg / g at the optimal HCl pretreatment conditions,indicating that HCl pretreatment could improve the saccharification efficiency of willow leaves.

关 键 词：柳树叶 HCl预处理 正交实验 响应曲面法 优化 

分 类 号：X72[环境科学与工程—环境工程]