巴戟天胚乳诱导愈伤组织及三倍体再生  

Endosperm Callus Induction and Triploid Plant Regeneration of Morinda officinalis How.

作　　者：姚焱[1] 孔曜 黄得凤 张旭霞 张英[2] 汪珍春[1] 张平[1] 

机构地区：[1]广州大学植物抗逆基因功能研究广州市重点实验室,广州大学生命科学学院,广东广州510006 [2]广州中医药大学中药学院,广东广州510006

出　　处：《种子》2015年第2期32-34,38,共4页Seed

基　　金：广东省科技计划项目(编号:2012 A 020602064);广州市科技计划项目(编号:2012 J 4300060);广州市属高校科研项目(编号:10 A 046);国家级大学生创新训练项目(编号:201211078025)

摘　　要：目的：利用巴戟天种子内胚乳为外植体进行离体培养获得三倍体。方法：选取未成熟巴戟天种子内胚乳,置于不同培养基上诱导愈伤组织和分化再生植株,并对植株倍性进行根尖染色体鉴定。结果：在MS＋2,4-D（2,4-二氯苯氧乙酸）2.0 mg/L＋6-BA（6-苄基嘌呤）1.0 mg/L培养基上诱导获得愈伤组织,诱导频率达58.9%。愈伤组织在MS＋6-BA1.0-2.0 mg/L＋IBA 0.1-0.5 mg/L分化培养基上分化出不定芽。不定芽在1/2 MS＋IBA 0.5 mg/L上诱导生根,生根率100%。再生植株根尖细胞染色体数目为2 n=3 x=33。结论：利用巴戟天胚乳培养获得三倍体是创造多倍体新种质的有效途径。Objective：To establish the effective method by in vitro culture endosperms to obtain the triploid plant of Morinda officinalis How. Methods ：The effects of plant growth regulators on inducing endosperm callus and plant regeneration of Morinda officinalis How. were studied. Results ： The optional inducing callus mediums was MS ＋ 2,4-D 2.0 mg/L ＋ 6-BA 1.0 mg/L, the callus induced rate was 58.9% ;the bud differentiation medium was MS ＋ 6-BA 1.0 - 2.0 mg/L ＋ IBA 0. 1 - 0.5 mg/L, the root medium was 1/2 MS ＋ IBA 0.5 mg/L, the root rate was 100%. The chromosome number of regenerated plant root tip cells was 2 n = 3 × = 33, a triploid plant. Conclusion： Culturing endosperms in vitro is an effective method to create new triploid germplasm.

关 键 词：巴戟天 胚乳培养 三倍体 

分 类 号：S336[农业科学—作物遗传育种] R282[农业科学—农艺学]