育成期水貂不同铜源相对生物学利用率和血清生化指标的研究  

A Study of Different Copper Sources Relative Bioavailability and Serum Biochemical Indices of Minks in Late Growing Period

作　　者：吴学壮[1,2] 张铁涛[2] 于淼[2] 刘志[1] 郭俊刚[1] 高秀华[1] 杨福合[2] 邢秀梅[2] 

机构地区：[1]中国农业科学院饲料研究所,北京100081 [2]中国农业科学院特产研究所,吉林省特种经济动物分子生物学省部共建重点实验室,长春130112

出　　处：《动物营养学报》2015年第2期485-494,共10页CHINESE JOURNAL OF ANIMAL NUTRITION

基　　金：国家自然资源平台专项"特种经济动物种质资源共享平台"(2005DKA21102)

摘　　要：本试验旨在研究饲粮不同铜源及水平对育成期水貂血清铜、锌和铁含量及血清氮代谢、脂类和抗氧化指标影响,以及水貂对不同铜源的相对生物学利用率。选择60日龄健康、体重接近的水貂240只,随机分成10组,每组24个重复,每个重复1只貂。试验采用3×3+1试验设计,对照组饲喂基础饲粮,试验组分别在基础饲粮中添加3种铜源(硫酸铜、碱式氯化铜、蛋氨酸铜)和3个铜添加水平(10、25、40mg/kg),预试期7d,正试期45d。结果表明:水貂血清铜含量随饲粮铜添加水平增加呈极显著线性增加(P=0.0004)。水貂血清总胆固醇(P=0.0015)、甘油三酯(P=0.0021)和高密度脂蛋白胆固醇(P=0.0101)含量随饲粮铜添加水平增加呈显著或极显著线性降低,然而水貂血清低密度脂蛋白胆固醇含量却未受饲粮铜添加水平的影响(P=0.5790)。水貂血清铜蓝蛋白和铜锌超氧化物歧化酶活性均随饲粮铜添加水平增加呈极显著线性升高(P=0.0001)。以血清铜蓝蛋白活性为指标,以硫酸铜为参比标准物(100%),碱式氯化铜和蛋氨酸铜的相对生物学利用率分别为109.93%和131.06%。以血清铜锌超氧化物歧化酶活性为指标,以硫酸铜为参比标准物(100%),碱式氯化铜和蛋氨酸铜的相对生物学利用率分别为111.01%和118.10%。由此可见,饲粮添加40mg/kg的铜(基础饲粮铜含量8.05mg/kg)能提高育成期水貂血清抗氧化酶的活性;育成期水貂3组铜源的相对生物学利用率为:蛋氨酸螯合铜>碱式氯化铜>硫酸铜。This experiment was conducted to study the effects of different copper sources and levels on serum copper, zinc and iron contents, nitrogen metabolism, lipid and antioxidant indices of minks in late growing pe-riod, and different copper sources relative bioavailability of minks. Two hundred and forty 60-day-old minks were randomly divided into 10 groups with 24 replicates per group and 1 mink per replicate. The experiment was used in a 3×3＋1 factorial experiment design, minks in the control group were fed a basal diet, and minks in other nine groups were fed basal diets supplemented with 3 sources （ copper sulfate, tribasic copper chloride and copper methionine） and 3 levels （10, 25, and 40 mg/kg）. The pre-test period lasted for 7 days, and the trial lasted for 45 days. The results showed as follows：the serum copper content of minks was significant linear increased with dietary copper supplemental level increased （ P=0.000 4） , the contents of total cholesterol （ P=0.001 5）, triglyceride （P=0.002 1） and high density lipoprotein cholesterol （P=0.010 1） in serum of minks were significant linear decreased with dietary copper supplemental level increased, but dietary copper supple-mental level had no significant effect on serum high density lipoprotein cholesterol content of minks （ P=0.579 0）. The activities of ceruloplasmin （CRE） and copper zinc superoxide dismutase （Cu-Zn SOD） in ser-um of minks were significant linear increased with dietary copper supplemental level increased （ P=0.000 1） . Compared with copper sulfate （ 100%） , the relative bioavailability of tribasic copper chloride and copper me-thionine were 109.93% and 131.06% based on serum CER activity, respectively. Compared with copper sul-fate （100%）, the relative bioavailability of tribasic copper chloride and copper methionine were 111.01% and 118.10% based on serum Cu-Zn SOD activity, respectively. The results indicate that dietary supplemented with 40 mg/kg copper （ copper content in basal diet

关 键 词：水貂 硫酸铜 碱式氯化铜 蛋氨酸铜 相对生物学利用率 血清生化指标 

分 类 号：S816.7[农业科学—饲料科学]