机构地区:[1]广东省农业科学院动物科学研究所,畜禽育种国家重点实验室,农业部华南动物营养与饲料重点实验室,广东省动物育种与营养公共实验室,广东省畜禽育种与营养研究重点实验室,广州510640
出 处:《动物营养学报》2015年第2期578-587,共10页CHINESE JOURNAL OF ANIMAL NUTRITION
基 金:国家肉鸡产业技术体系项目(CARS-42);公益性行业(农业)科研专项(200903006)
摘 要:本研究旨在通过研究饲粮铜添加水平对黄羽肉鸡生长性能、免疫器官发育、抗氧化性能、肝脏和血清铜含量等的影响,以探讨1~21日龄黄羽肉鸡铜的营养需要量。选用1日龄健康、发育良好的快大型岭南黄羽肉公雏鸡1440只,根据体重随机分为6个组,每组6个重复,每个重复40只鸡,组1(对照组)饲喂基础饲粮(2.80 mg/kg铜),组2、3、4、5、6饲粮在基础饲粮中分别添加4、8、16、32、64 mg/kg铜。试验期21 d。结果表明:本试验条件下,1)饲粮添加4和64 mg/kg铜显著提高试鸡平均日采食量( P<0.05),饲粮中铜添加水平对末重、平均日增重和料重比无显著影响( P>0.05)。2)与对照组相比,8 mg/kg组法氏囊指数显著升高( P<0.05),而饲粮铜添加水平对胸腺和脾脏指数无显著影响( P>0.05)。3)与对照组相比,8、16、32和64 mg/kg组血清和肝脏铜锌超氧化物歧化酶(CuZnSOD)活性显著升高(P<0.05),各组间血清和肝脏丙二醛含量均无显著差异(P>0.05);添加16、32、64 mg/kg铜显著降低血清铜蓝蛋白活性(P<0.05)。4)与对照组相比,16、32和64 mg/kg组血清铜含量显著降低(P<0.05),铜添加水平对肝脏铜含量和肝脏金属硫蛋白含量影响不显著( P>0.05)。综合考虑各项指标,为获得较好生长性能和免疫器官发育、抗氧化性能,1~21日龄快大型黄羽肉鸡饲粮铜适宜添加水平为8 mg/kg,加上基础饲粮铜含量2.80 mg/kg,则该阶段黄羽肉鸡铜需要量为10.80 mg/kg;以血清CuZnSOD活性为依据,通过非线性回归分析估测得到1~21日龄黄羽肉鸡铜需要量为13.38 mg/kg。This experiment was conducted to investigate the effects of dietary copper supplemental level on growth performance, immune organ development, antioxidative property, copper content in serum and liver of yellow-feathered broilers etc. , then to estimate the dietary copper requirement of yellow-feathered broilers aged from 1 to 21 days. A total of 1 440 twenty-two days old healthy, well-developmental, fast large male Lingnan yellow-feathered broilers were randomly assigned to six groups. Each group consisted of six replicates with 40 birds per replicate. Diets of these groups included the purified basal diet supplemented with different levels of copper to ensure dietary copper levels up to 2.80 ( control) , 6.80, 10.80, 18.80, 34.80, 66.80 mg/kg, re-spectively. The contents of other nutrients in diets were consistent. The experiment lasted for 21 days. Results showed as follows: under the experiment condition, 1 ) the average daily feed intake of broilers in 4, 64 mg/kg groups were significantly higher than those in the other groups ( P〈0.05) , but there were no signifi-cant differences in the final weight, average daily gain, ratio of feed to gain among all groups ( P〉0.05) . 2) Compared with the control group, diet supplemented with 8 mg/kg copper significantly improved index of bur-sa of Fabricius ( P〈0.05) , but copper supplemental level did not significantly affect the spleen and thymus in-dexes ( P〉0.05) . 3) The CuZn-superoxide dismutase ( CuZnSOD) activity in serum and liver in 8, 16, 32 and 64 mg/kg groups was significantly higher than that in the control group ( P〈0.05) . Dietary copper supple-mental level did not significantly affect the content of malondialdehyde in serum and liver of broilers ( P〉0.05) . Diet supplemented with 16, 32 and 64 mg/kg copper significantly decreased the ceruloplasmin activity in serum ( P〈0.05) . 4) Compared with the control group, diet supplemented with 16, 32 and 64 mg/kg cop-per significantly decreased the coppe
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