化学发光免疫法与放射免疫法检测血清促甲状腺激素受体抗体的对比研究  

Comparison of a chemiluminescent immunoassay with radioimmunoassay for detection of serum thyrotropin receptor antibody

作　　者：杨开洪 邹辉祥 曾庆栈 

机构地区：[1]广东省阳江市中医医院,529500

出　　处：《实用医技杂志》2015年第2期122-123,共2页Journal of Practical Medical Techniques

摘　　要：目的比较化学发光免疫法(CLIA)与放射免疫法(RIA)2种方法测定血清促甲状腺激素受体抗体(TRAb)水平的差异性,探讨CLIA检测TRAb的临床可行性。方法收集60例临床受检血清样本,并根据临床检查结果分为Graves病组和健康对照组,采用CLIA和RIA同时检测2组的血清TRAb水平。结果采用2种检测方法测定的Graves病组的TRAb水平均高于健康对照组(P<0.05)。2种检测方法测定的TRAb水平具有高度的相关性(r=0.990,P<0.01)。2种检测方法的阳性符合率为97.0%,阴性符合率为94.4%,总体符合率为96.1%。2种检测方法的阳性率的差异无统计学意义(P>0.05)。结论在血清TRAb水平的检测上,CLIA与RIA没有明显的差异性,同时CLIA具有灵敏度高、特异性强、自动化高,速度快、无放射性等优点,便于临床应用。Objective To investigate the difference between chemiluminescent immunoassay （CLIA） and radio- immunoassay（RIA） for detection of serum Thyrotropin receptor antibody（TRAb）, and the clinical feasibility of detecting serum TRAb using CLIA. Methods All 60 patients were classified into Graves＇ Disease and healthy control groups according to the clinical examination, and serum TRAb of which were detected using both CLIA and RIA. Results Serum TRAb of Graves＇ disease group was higher than that of health control group using both CLIA and RIA （P〈0.05）. The levels of serum TRAb detected by CLIA and RIA are highly correlated （r=0.990, P〈0.01 ）. The positive coincidence rate, negative coincidence rate, and overall coincidence rate for testing serum TRAb by CLIA and RIA were 97.0%, 94.4%, and 96.1% respectively, and the positive rates had no statistically significance between the two methods（P〉0.05）. Conclusion There are no significant differences for testing serum TRAb between CLIA and RIA, while the CLIA method has advantages of high sensitivity and specificity, high speed and automation, and non-radioactive, to facilitate the clinical application.

关 键 词：化学发光免疫法 放射免疫法 促甲状腺激素受体抗体 

分 类 号：R446.6[医药卫生—诊断学]