急性一氧化碳中毒对大鼠少突胶质前体细胞的影响  

Effect of acute carbon monoxide poisoning on oligodendrocyte precursor cells in rats

作　　者：孙瑞佼 郭大志[1,2] 李航[1,2] 李铭鑫[1,2] 胡慧军[1,2] 潘晓雯[1,2] 

机构地区：[1]第二军医大学海军临床医学院 [2]海军总医院高压氧科,北京100048

出　　处：《中国康复》2015年第1期10-13,共4页Chinese Journal of Rehabilitation

基　　金：海军总医院创新培育基金资助(CXPY201303)

摘　　要：目的:通过制备急性一氧化碳中毒(acute carbon monoxide poisoning,ACOP)大鼠模型,观察大鼠中毒后中枢神经系统髓鞘改变,以及髓鞘标志物髓鞘碱性蛋白(myelin basic protein,MBP)和少突胶质前体细胞(oligodendrocyte precursor cells,OPCs)特异性细胞标志物硫酸软骨素蛋白多糖NG2在中毒后表达的变化,为研究ACOP后中枢神经系统髓鞘脱失的机制提供实验依据。方法:24只雄性健康SD大鼠,随机分为对照组和染毒组各12只。染毒组利用分次腹腔注射CO制作急性中毒模型,对照组注射空气。于造模后1d、3d取材,采用免疫组织化学染色和Western Blot方法检测中毒后中枢神经系统髓鞘的损伤和MBP、NG2表达的变化。结果:免疫组织化学染色结果显示,与对照组比较,染毒组染毒1d后MBP、NG2表达即减少,3d时进一步降低(P<0.05);Western Blot结果显示,染毒组染毒1d后MBP、NG2表达即减少,3d时进一步降低(P<0.05)。结论:ACOP后大鼠发生中枢神经系统脱髓鞘以及OPCs损伤。Objective：To observe the changes in myelin and expression of myelin basic protein（MBP）and NG2 in the brain tissue of rats through the establishment of the acute carbon monoxide poisoning（ACOP）model,and to provide experimental evidence for the mechanism of demyelination of the central nervous system after ACOP.Methods：Twenty-four male rats were divided into the control group（n=12）and the intoxication group（n=12）randomly.Animals in the intoxication group were intraperitoneally injected with pure CO repeatedly,and those in the control group with air by the same protocol.The brain tissues were taken from the animals of the two groups at 1st and3 rd day after the establishment of the model.Changes in myelin were observed by immunohistochemical staining,and the expression of MBP and NG2 was detected using immunohistochemistry and Western blot.Results：Immunohistochemistry demonstrated that the expression levels of MBP and NG2 began to decrease at 1st day after ACOP,and there was statistically significant difference in the integral optical density（IOD）level of MBP and NG2 as compared with the control group at 3rd day after ACOP（P〈0.05）.Results of Western blot showed that as compared with the control group,the expression levels of MBP and NG2 were decreased at 1st day after ACOP,and decreased further at 3rd day（P〈0.05）.Conclusion：Demyelination and damage of oligodendrocyte precursor cells occurred in the brain tissue of rats after ACOP.

关 键 词：急性一氧化碳中毒 脱髓鞘 髓鞘碱性蛋白 硫酸软骨素蛋白多糖NG2 少突胶质前体细胞 

分 类 号：R595.1[医药卫生—内科学]