草鱼促性腺激素受体的克隆及表达分析  

Molecular Gene Cloning and Expression of Gonadotropin Receptors from Ctenopharyngodon idella

作　　者：佘冬莲 陈文娟[1] 王艳杰[1] 周毅[1] 刘琼[1] 李建中[1] 

机构地区：[1]湖南师范大学生命科学学院,中国湖南长沙410081

出　　处：《生命科学研究》2015年第1期39-43,共5页Life Science Research

基　　金：国家自然科学基金项目(31272261;31072199);留学回国人员科研启动基金资助项目(教外司留(2011)1568号)

摘　　要：利用RT-PCR技术从草鱼性腺中克隆出两种促性腺激素受体基因(FSHR和LHR),采用N-J法构建了系统进化树。通过RT-PCR及Real-time PCR技术对FSHR和LHR在成体草鱼不同组织中的时空表达进行分析,发现FSHR和LHR在草鱼卵巢、精巢中表达明显,表达量随着卵母细胞的发育逐渐增高,以上结果为进一步探讨GTHR在鱼类生殖周期中的生理功能奠定了基础。Two types of gonadotropin receptors（FSHR and LHR） were cloned from the gonads of grass carp by RT-PCR. The phylogenetic tree of GTHR between different species by N-J method was constructed. The temporal and spatial expression of different tissues in adult grass carp by RT-PCR and Real-time PCR was analyzed. The result indicated that FSHR and LHR were much higher expressed in gonads, and the expression level was significantly increased in mature oocytes. These results laid a foundation for the further study on GTHR reproductive functions in fish.

关 键 词：草鱼 促性腺激素受体 基因克隆 表达分析 

分 类 号：Q785[生物学—分子生物学] Q786