晶体生长过程实时监测系统  

On the Real-Time Monitoring System of Crystal Growth Process

作　　者：赵静[1,2] 杨翀[1,3] 孔繁羽 缪泓[1] 

机构地区：[1]中国科学技术大学中国科学院材料力学行为和设计重点实验室,安徽合肥230026 [2]安徽大学电气工程与自动化学院,安徽合肥230601 [3]中国工程物理研究院总体工程研究所,四川绵阳621900 [4]四川农业大学理学院,四川雅安625014

出　　处：《实验力学》2015年第1期59-66,共8页Journal of Experimental Mechanics

基　　金：国家重点基础研究发展计划资助项目(2012CB937500);国家自然科学基金项目(11372182;11472267;11072235)资助

摘　　要：实时监测晶体生长过程对研究晶体生长动力学以及调控晶体生长过程有着很重要的意义。结合干涉技术、相移技术以及时间序列位相提取技术,建立了一个晶体生长过程实时监测系统。该系统主要由三条光束组成,通过设定快门参数组成不同的Mach-Zehnder干涉光路,可同时用于无机盐晶体和蛋白质晶体生长过程的实时定量监测。利用该系统及时间序列位相提取方法,对NaCl晶体在自然蒸发情况下的生长过程以及溶菌酶蛋白悬滴法结晶过程进行了研究,对该系统和方法在研究晶体结晶过程中的可行性及优势进行了验证。结果表明,该系统不仅可以获得晶体结晶过程中的实时绝对浓度、过饱和度等晶体生长动力学参数;而且对于蛋白质结晶过程,快门系统的引入解决了蛋白溶液多浓度场叠加的分离问题,在一个实验周期内获得了溶菌酶蛋白结晶过程中的实时蛋白浓度演变、实时沉淀剂浓度变化等结晶动力学参数。这些关键参数为下一步探索结晶条件对晶体质量的影响、分析蛋白结晶相图以及优化蛋白质晶体质量提供了定量数据基础。Real-time monitoring crystal growth process is proved to be very important in the study of crystal growth kinetics and the optimization of crystal growth process.A real-time monitoring system is established and presented in this paper,based on the combining of optical interferometry,phaseshift technique and time series phase retrieval method.The system is mainly composed by three beams and can compose different Mach-Zehnder optical interferometer by setting the shutter parameters.It can be simultaneously used in real-time quantitative monitoring of inorganic crystal and protein crystal growth process.Using this system and time series phase retrieval method,NaCl crystal growth process in the case of natural evaporation and Lysozyme protein crystallization process by hanging drop method were investigated,respectively.The feasibility and advantages of the system and methods adopted in study of crystal crystallization process were verified.Results show that the crystal growth kinetics parameters such as real-time absolute concentration and supersaturation during enitre NaCl crystallization process can be obtained.In addition,for protein crystallization process,the realtime protein concentration and precipitation concentration are separated from multi-concentration field superposition in one experimental period.These key parameters provide quantitative data foundation for protein crystallization phase diagram analysis and the crystal quality optimization.

关 键 词：蛋白质结晶 时间序列位相分析 相移干涉 绝对浓度 

分 类 号：O34[理学—固体力学] O78[理学—力学]