双波长HPLC同时测定氮沉降处理下杜仲皮和叶中的5种成分  

Determination of five active components in Eucommia uimoides under simulated experiment of nitrogen deposition by dual-wavelength HPLC

作　　者：哀建国[1] 杜江丽[2] 周爱存[2] 金松恒[2] 宋新章[2] 

机构地区：[1]浙江农林大学信息工程学院,浙江临安311300 [2]浙江农林大学林业与生物技术学院,浙江临安311300

出　　处：《浙江农林大学学报》2015年第1期60-66,共7页Journal of Zhejiang A&F University

基　　金：浙江省自然科学基金资助项目(Y3110393)

摘　　要：建立双波长高效液相色谱法（HPLC）同时测定模拟氮沉降处理下杜仲Eucommia uimoides皮和叶中松脂醇二葡萄糖苷、桃叶珊瑚苷、绿原酸、京尼平苷酸和京尼平苷等5种活性成分含量的方法。采用Agilent ZORBAX SB-C18柱（250.0 mm×4.6 mm,5μm）,以乙腈（A）-2.0 g·kg-1磷酸水溶液（B）进行梯度洗脱（0~12 min,10%A;12~25 min,13%A;25~50 min,30%A;50~65 min,60%A）;流速为0.8 m L·min-1;检测波长203 nm,240 nm;柱温30℃。结果表明：松脂醇二葡萄糖苷、桃叶珊瑚苷、绿原酸、京尼平苷酸、京尼平苷等在各自的线性范围内与其峰面积积分值呈良好的线性关系。整个实验期间,杜仲皮和叶中5种成分含量对施氮处理产生了不同的响应,且随季节在变动。该方法能准确、快速地对杜仲皮和叶中多种有效成分同时进行测定,可较好反映浙江高氮沉降区特色名贵药材杜仲皮和叶中多种有效成分的变化。A method for determination of pinoresinol diglucoside, aucubin, chlorogenic acid, geniposidic acid and geniposide in Eucommia uimoides under simulated experiment of nitrogen deposition by dual wavelength HPLC was established. Dual-wavelength HPLC was adopted performing on Agilent ZORBAX SB-CI8 column （250 mm×4.6 mm,5μm） with a mobile phase of acetonitrile （A） -0.2% phosphoric acid （B） at flow rate of 0.8 mL min-1. The gradient elution program was as follows： 0-12 min, 10%A; 12-25 min, 13%A; 25-50 min, 30% A; 50-65 min, 60% A. The detection wavelength was set at 203 nm for pinoresinol diglucoside and aueubin, and at 240 nm for ehlorogenic acid, geniposidic acid and geniposide. The column temperature was 30 ℃. Results showed that pinoresinol diglucoside, aueubin, chlorogenic acid, geniposidie acid and geniposide all had a good linear relationship with their peak area values respectively. The five ingredients from bark and leaves of Eucommia uimoides had different response to the nitrogen treatments, and changing in the seasons. The method can be used for simultaneous determination of ingredients in Eucommia uimoides, and can be successfully applied to reflect the content changing of the compounds.

关 键 词：经济林学 杜仲 模拟氮沉降 药用成分 双波长高效液相色谱法 

分 类 号：S759.3[农业科学—森林经理学]