野百合碱注射建立大鼠肺动脉高压模型的方法  

Monocrotaline injection in establishing rat model of pulmonary hypertension

作　　者：李松华[1] 石仁华 朱琴[2] 史承勇[1] 杜贺[1] 赵仙先[1] 陈少萍[1] 秦永文[1] 

机构地区：[1]第二军医大学长海医院心血管内科,上海200433 [2]第二军医大学翔殷路干休所,上海200433

出　　处：《解放军医学院学报》2015年第2期171-174,共4页Academic Journal of Chinese PLA Medical School

基　　金：上海市科学技术委员会科研计划项目(13ZR1409000);上海市卫生局局级科研项目(2012461);长海医院"1255"学科建设计划资助项目(CH125531702)~~

摘　　要：目的探讨野百合碱注射建立大鼠肺动脉高压模型的方法和关键点。方法 SPF级雄性SD大鼠30只,随机分为实验组和对照组各15只。实验组大鼠颈背部皮下注射野百合碱50 mg/kg;对照组皮下注射0.9%氯化钠注射液1 ml。3周后大鼠行右心导管检查、病理检查和免疫组化检查。结果实验组大鼠表现为进行性右心功能衰竭,死亡2只,平均肺动脉压力、右心室收缩压均明显高于对照组[(40.05±1.78)mm Hg vs(18.63±1.80)mm Hg(1 mm Hg=0.133 k Pa),(62.99±2.03)mm Hg vs(28.04±2.26)mm Hg,P均<0.05]。病理切片HE染色,实验组大鼠肺血管中膜明显增生,管腔狭窄,中膜管壁厚度百分比、中膜管腔面积百分比、右心室肥厚指数[RV/(LV+IVS)]均明显大于对照组[(0.292±0.064)vs(0.106±0.026)、(0.0495±0.092)vs(0.200±0.045)、(0.473±0.041)vs(0.295±0.035),P均<0.05]。实验组大鼠肺血管增殖细胞核抗原免疫组化IA值明显高于对照组[(39.5±8.7)vs(18.5±5.3),P<0.05]。结论经颈背部皮下注射野百合碱建立大鼠肺动脉高压动物模型是一种操作简单、有效可靠的方法,把握几个关键点可以提高建模成功率。Objective To investigate the method and key points of monocrotaline injection in establishing pulmonary hypertension model of rats. Methods Thirty Sprague dawley （SD） male rats of SPF grade were randomly divided into two groups： experiment group （n=15） and control group （n=15）. The experimental rats received subcutaneous injection of monocrotaline accounting for 50 mg/kg, and rats in control group received subcutaneous injection of 0.9% sodium chloride accounting for 1 ml. Three weeks later, the rats underwent fight heart catheterization, pathology and immunohistochemlstry. Results Rats in experimental group showed progressive fight heart failure with 2 rats died. The mean pulmonary artery pressure and fight ventricular systolic pressure in experimental rats were significantly higher than control group [（40.05 ± 1.78） mmHg vs （18.63 ± 1.80） mmHg （I mmHg=0.133 kPa）, （62.99 ± 2.03） mmHg vs （28.04± 2.26） mmHg, P 〈 0.05]. HE staining showed rats in the experimental group with pulmonary vascular membrane hyperplasia and stenosis, and WT%, WA%, RV/（LV ＋ IVS） in rats of experimental group were significantly higher than control group [（0.292 ± 0.064） vs （0.106 ± 0.026）, （0.0495 ± 0.092） vs （0.200 ±0.045）, （0.473 ± 0.041） vs （0.295 ±0.035）, P 〈 0.05]. IA value of pulmonary vascular PCNA immunohistochemistry in experimental group was significantly higher than control group [（39.5 ± 8.7） vs （18.5± 5.3）, P 〈 0.05]. Conclusion Monocrotaline injection in establishing rat model of pulmonary hypertension is a simple, effective and reliable method, and the modeling success rate can be improved with a few key points.

关 键 词：肺动脉高压 大鼠 野百合碱 动物模型 

分 类 号：R692[医药卫生—泌尿科学]