杨树组织特异性强启动子的筛选  

Experimental Analysis of the Effect of Processing and Usage to Semen Sinapis and Raphani Couplet Medicines on Total Component~ ahd Sinapine Dissolutions

作　　者：李丽[1] 翟文君[1] 吕文海[1] 

机构地区：[1]山东中医药大学药学院,山东济南250355

出　　处：《辽宁中医药大学学报》2015年第3期40-42,共3页Journal of Liaoning University of Traditional Chinese Medicine

基　　金：国家自然科学基金项目（No.31000305）; 河北农业大学林学学科创新基金项目（LXXK2014-1）

摘　　要：为了筛选到具有木质部组织特异性并有较强功能的启动子,将河北林木种质资源与森林保护重点实验室前期已获得的MDCes A启动子、JCes AP启动子、DMDCes AP启动子（含2个串联MDCes AP启动子）融合GUS报告基因的p MDCes AP-GUS、p JCes AP-GUS、p DMDCes AP-GUS这3个植物表达载体,通过农杆菌介导转化杨树,对转化后的杨树进行GUS活性的定性及荧光定量检测,以比较3个启动子在转基因植物中的表达能力和水平。筛选到的组织特异启动子与抗天牛基因融合,将提高毒蛋白在相应部位的表达量,这对天牛类蛀干害虫的防治具有重大意义。Objective： To explore the effects of processliig and usage to Laifuzi （Radish Seed） and Jiezi（Mustard Seed） on total components and sinapine disolutlns. Methods ： Chinese Pharmacopoeia methodwas applied Io measure water and alcohol extracts arid the fatty oil contents. The content of sinapinc wasdetermined by HPLC in water decoction. The composition of fatty acids waanalyzed by GC-Mb. ResuhAll ingredients＇ dissolutions increased slightly by processing, trushing of drug effe.cts soluhilizatitm wasignificant on each component. The difference between siugle-decotion and mixed-decoction oH measurillgpart stripping was not obvious. Conclusion： Clinical applicati,m of traditional Chinese medicine must inaccordance with the processing and the reasonable use to ensure its effectiveness.

关 键 词：木质部特异启动子 筛选 GUS报告基因 荧光定量检测 

分 类 号：R283[医药卫生—中药学]