普罗布考对高同型半胱氨酸血症兔主动脉粥样硬化及5-脂氧酶表达的影响  

Effect of Probucol on atherosclerosis induced by hyperhomocysteinemia and the expression of 5-lipoxygenase in rabbits

作　　者：吴乐[1] 刘子建[2] 黎红华[1] 武强[1] 

机构地区：[1]广州军区武汉总医院神经内科,武汉430070 [2]华中科技大学同济医学院解剖学系,武汉430030

出　　处：《中国组织化学与细胞化学杂志》2015年第1期23-26,共4页Chinese Journal of Histochemistry and Cytochemistry

基　　金：国家自然科学基金资助(81100867)

摘　　要：目的观察普罗布考对高同型半胱氨酸(Hcy)血症兔主动脉粥样硬化(AS)及5-脂氧酶(5-LOX)表达的影响。方法将24只雄性新西兰大耳白兔分成3组:对照组给予基础饲料喂养;模型组在基础饲料中加入1%的蛋氨酸;治疗组给予高蛋氨酸饮食,同时用普罗布考灌胃(1g/d);每组8只,饮食量200g/d。第20周末处死动物,快速打开腹腔剥离腹主动脉。用逆转录聚合酶链法(RT-PCR)和Western印迹法(Western-blotting)技术检测动脉血管中5-LOX的mRNA及蛋白表达,光镜下观察主动脉病理形态学变化。结果主动脉病理形态学检查显示治疗组病变明显轻于模型组;治疗组主动脉5-LOX的mRNA及蛋白表达水平明显低于模型组。结论普罗布考能抑制主动脉5-LOX的mRNA及蛋白表达,减轻内膜增生程度,该作用可能是普罗布考抗AS的机制之一。Objective To observe the effects of Probucol on atherosclerosis induced by hyperhomocysteinemia and the expression of 5-lipoxygenase(5-LOX)in rabbits.Methods Twenty-four male rabbits were randomly divided into three groups.The control group was fed on common food;the homocysterine(Hcy)model group was fed on high-methionine food containing 1% methionine;the treatment group with highmethionine food and Probucol 1g/d perfused into stomach.Eight rabbits in each group were fed received200 g food per day.The aorta was removed at the end of the protocol for the investigation of 5-LOX expression in aorta by RT-PCR and Western-blotting.The sections were made for light microscopy to observe the vascular pathomorphologic changes 20 weeks after experiment.Results The mRNA and protein expression of 5-LOX was down-regulated in the treatment group.Aortic lesion was attenuated compared with the model group.Conclusion Probucol reverses the aortic lesion induced by Hcy.The mechanism may be related to its suppressive effect on the mRNA and protein expression of 5-LOX.

关 键 词：普罗布考 同型半胱氨酸 动脉粥样硬化 5-脂氧酶 

分 类 号：R322.121[医药卫生—人体解剖和组织胚胎学]