含肝细胞生长因子水凝胶修复家兔股骨颈部缺损  

Hydrogel containing hepatocyte growth factor promotes the repair of femoral neck defects in rabbits

作　　者：郭林[1] 甄瑞鑫[1] 赵德伟[1] 田丰德[1] 于晶[1] 朱瑞萍[1] 

机构地区：[1]大连大学附属中山医院,辽宁省大连市116001

出　　处：《中国组织工程研究》2014年第52期8470-8473,共4页Chinese Journal of Tissue Engineering Research

摘　　要：背景:肝细胞生长因子可促进骨组织再生,在骨组织修复方面有着巨大的潜力,但是在体内较短的半衰期限制了其在临床的应用。目的:观察含有肝细胞生长因子的半合成细胞外基质样水凝胶体内促进股骨颈骨缺损修复的作用。方法:取12只兔制作双侧股骨颈骨缺损模型,采用自体配对对比,左侧为对照侧不对骨缺损做任何处置,右侧为实验侧植入含有肝细胞生长因子的半合成细胞外基质样水凝胶。结果与结论:苏木精-伊红染色见2周时实验侧缺损修复区血管分布较对照侧均匀;4周时实验侧缺损区均匀填充新生骨小梁,而对照侧由外向内骨小梁形成减少;8周时实验侧缺损区皮质骨形成,并且骨髓腔再通,有骨髓细胞填充,对照侧仍有粗大骨痂填充缺损区,堵塞髓腔。钼靶X射线观测到8周时,实验侧难以区分正常骨质与新生骨质交界线,而对照侧缺损区与周围骨质分界明显。证实含有肝细胞生长因子的半合成细胞外基质样水凝胶可以促进家兔股骨颈部骨缺损的骨组织修复。BACKGROUND： Hepatocyte growth factor can promote bone regeneration and has a great potential in bone repair, but its shorter half-life in vivo limits its clinical application. OBJECTIVE： To observe the promoting effect of semisynthetic extracellular matrix-like hydrogel containing hepatocyte growth factor on the repair of femoral neck defects in vivo. METHODS： Twelve rabbits were taken to make bilateral femoral neck defect models. Based on autologous paired comparison, the left side was control side without treatment, and the right side served as experimental side undergoing implantation of semisynthetic extracellular matrix-like hydrogel containing hepatocyte growth factor. RESULTS AND CONCLUSION： Hematoxylin-eosin staining showed more uniform distribution of vessels in the defect area of the experimental side than the control side after 2 weeks; after 4 weeks, new bone trabeculae were full of the defect area of the experimental side, but bone trabeculae from inside to outside formed less in the control side; after 8 weeks, cortical bone formation appeared in the experimental side, with the reascularization of the bone marrow cavity that was filled with the bone marrow cells, while in the controlled side, thick calluses were still full of the defect area which blocking the bone marrow cavity. Mammography X-ray observations showed that at 8 weeks, it was difficult to distinguish the border between normal bone and new bone in the experimental side, but the border was still obvious in the controls side. It is confirmed that semisynthetic extracellular matrix-like hydrogel containing hepatocyte growth factor is beneficial to repairing femoral neck defects of rabbits.

关 键 词：组织构建 缓释材料 肝细胞生长因子 细胞外基质材料 组织工程 水凝胶 骨缺损 修复 兔 半合成细胞外基质样水凝胶 

分 类 号：R318[医药卫生—生物医学工程]