Tunel法检测海藻酸钙敷料对成纤维细胞凋亡的实验研究  

Study on the fibroblast apoptosis of calcium alginate dressing by the Tunel method

作　　者：汪涛[1] 赵珺[1] 刘芳[2] 袁子茗[1] 张健[1] 张震[1] 顾其胜 贾伟平[1] 

机构地区：[1]上海交通大学附属第六人民医院血管外科,上海200233 [2]上海交通大学附属第六人民医院内分泌科,上海200233 [3]上海其胜生物材料研究所,上海201100

出　　处：《中国现代普通外科进展》2014年第12期930-933,共4页Chinese Journal of Current Advances in General Surgery

摘　　要：目的 :探讨海藻酸钙敷料诱导成纤维细胞(L-929)的凋亡情况。方法:将成纤维细胞(L-929细胞)与海藻酸钙材料共培养72h作为实验组,另设空白培养液作为对照组,采用原位标记法(Tunel法)对细胞死亡类型进行检测,并在正置荧光显微镜下观察细胞凋亡情况,计算凋亡率。结果:两组均出现凋亡细胞,且随培养时间呈现持续升高趋势,实验组凋亡检出率与对照组相比无明显差异。结论:海藻酸钙敷料无明显诱导细胞的凋亡,具有良好生物相容性,有望成为良好的新型伤口敷料。Objective： To obtain the fibroblast cells（L929）apoptosis induced by the calcium alginate dressing in vitro. Methods： The fibroblast cells（L929）co-cultured with calcium alginate dressing for 72 h as experimental group, and the blank culture solution as the control group. Terminal uridine nick-end labeling（Tune）lmethod was used to identify the pattern of cell death, then the apoptosis rate of fibroblast in each group were calculated under the fluorescence microscope. Results：The results showed that the apoptotic cells were appeared in both groups, and increased along with the incubation time. However, the apoptosis rate in each group showed no significant differences.Conclusion： Calcium alginate dressing did not induce the apoptosis of fibroblast cells. It possesses a good cytocompatibility and has the potential value as a appropriate wound dressing.

关 键 词：海藻酸钙敷料 TUNEL法 细胞凋亡 

分 类 号：R619.6[医药卫生—外科学]