替米沙坦对高血压患者外周血T淋巴细胞Kv1.3钾通道表达的抑制作用  

The inhibitory effect of telmisartan on voltage-dependent potassium channel expression in peripheral blood T lymphocytes of patients with hypertension

作　　者：张邱兵 苟芳[2] 张源明[3] 贺集贤[1] 何元军[1] 袁庆艳 章桂铭 

机构地区：[1]广元市中心医院心内科,四川广元628000 [2]广元市中心医院内分泌科,四川广元628000 [3]新疆医科大学第一附属医院心脏中心

出　　处：《中华高血压杂志》2014年第12期1162-1166,共5页Chinese Journal of Hypertension

基　　金：国家自然科学基金(81160039)

摘　　要：目的研究替米沙坦对高血压患者T淋巴细胞电压依赖性钾通道(Kv1.3)mRNA和蛋白表达的影响。方法于2012年8月至12月在新疆医科大学第一附属医院高血压门诊随机入选未经降压药物治疗的新疆哈萨克族高血压患者30例,随机分为空白对照组、替米沙坦组及4-氨基吡啶(钾通道阻断剂)组3组,每组10例,男女各半。采用免疫磁珠法分离各组患者外周血T淋巴细胞进行体外培养活化增殖,给予相应分组处理[空白对照组不作任何处理,替米沙坦和4-氨基吡啶溶解后分别加入替米沙坦组和4-氨基吡啶组培养液],继续培养0、24、48h,并使用实时荧光定量PCR技术和Western blot检测哈萨克族高血压患者T淋巴细胞Kv1.3钾通道mRNA和蛋白的表达变化。结果替米沙坦组及4-氨基吡啶组T淋巴细胞Kv1.3钾通道mRNA和蛋白的表达较空白对照组减少(均P<0.05);替米沙坦对其mRNA表达的抑制率分别为50.0%、73.5%,对其蛋白表达的抑制率分别为49.3%、67.9%。结论替米沙坦能有效抑制高血压患者T淋巴细胞Kv1.3钾通道mRNA和蛋白的表达。Objective To study the inhibitory effect of telmisartan on the voltage-dependent potassium channel（Kv1.3）expression in peripheral blood T lymphocytes of patients with hypertension. Methods Thirty Xinjiang Kazakh hypertensive patients without antihypertensive therapy were randomly selected from hypertensive clinic of the First Affiliated Hospital of Xinjiang Medical University from August to December 2012,and the patients were randomly assigned to control,telmisartan or 4-aminopytidine（potassium channel blocker）group（n=10each group,5males and 5females）. Peripheral blood T lymphocytes isolated by magnetic cell sorting were first cultured for activation and proliferation in vitro,and then incubated for 0,24,or 48 hrespectively after telmisartan or 4-aminopytidine treatment. Real-time quantitative PCR（RT-PCR）and Western Blot were used to detect the Kv1.3channel mRNA and protein expression. Results The mRNA and protein expressions of the Kv1.3in T lymphocytes in telmisartan and 4-aminopytidine group were significantly reduced compared to those at the same time points of control group（all P〈0.05）.In T lymphocytes after 24 hand 48htreatment with telmisartan,inhibition rates of Kv1.3mRNA expression were 50.0%,73.5%,and the protein expression were 49.3%,67.9%,respectively.Conclusion Telmisartan could effectively inhibit the Kv1.3potassium channel mRNA and protein expression in T lymphocytes of patients with hypertension in Xinjiang Kazakh.

关 键 词：高血压 T淋巴细胞 Kv1.3钾通道 替米沙坦 

分 类 号：R544.1[医药卫生—心血管疾病]