高效液相体积排阻色谱法分析黄秋葵多糖的单糖组成  

Analysis of Monosaccharide Composition in Okra Polysaccharides by High Performance Liquid Chromatography

作　　者：裘雅渔[1] 李巧玲[1] 程和勇[2] 

机构地区：[1]浙江大学化学系,浙江杭州310027 [2]杭州师范大学材料系,浙江杭州310058

出　　处：《实验室研究与探索》2014年第12期27-30,共4页Research and Exploration In Laboratory

基　　金：浙江省科技厅测试基金项目(2012C37070)

摘　　要：采用高效液相(HPSEC-RID)体积排阻色谱法,建立了7种常见单糖的分离模式,成功地用于黄秋葵多糖的单糖组成分析。应用体积排阻色谱法,色谱柱为Polyspher CH PB(7.8 mm×300 mm,made in Germany),流动相为去离子水,柱温80℃,示差折光检测器(检测器温度40℃),流速0.6 ml/min内标为丙三醇。根据建立的方法测定,黄秋葵多糖由木糖、甘露糖、鼠李糖、葡萄糖、果糖、半乳糖及阿拉伯糖7种单糖组成,其摩尔比分别为0.57∶1.00∶0.53∶3.50∶1.75∶1.25∶1.70。该HPSEC-RID方法是简单、快速、灵敏、方便的分析技术,具有良好的重复性,可用于黄秋葵多糖的单糖组成的质量控制。A method of analysis the monosaccharide composition of Okre polysaccharides with good reproducibility is established in thhi paper. The method is to base on HPSEC-RID method and chromatographic conditions that inc1 ude Polyspher CH PB（ 7. 8 mm × 300 mm,made in Germany） column. The mobile phase is pure water at 40℃ of the column and the RID detector is 80℃. The method of HPSEC-RID analysis showed that Okra polysaccharides consists of xylose,mannose,rhamnose,glucose,fructose,galactose and arabonose with the molar ratios of 0. 57 ∶ 1. 00 ∶ 0. 53 ∶ 3. 50 ∶1. 75 ∶ 1. 25∶ 1. 70. It was proved that the HPSEC-RID method is simple,rapid,sensitive,convenient,and can be applied to the quality control of the monosaccharide composition of Okra polysaccharides.

关 键 词：黄秋葵多糖 单糖组成 高效液相体积排阻色谱法 

分 类 号：R284.1[医药卫生—中药学]