黄连解毒汤对小鼠S_(180)肉瘤血管生成抑制作用的试验研究  

Experimental study of Huanglianjiedu decoction on inhibiting S_(180) sarcoma angiogenesis mice

作　　者：孙燕[1] 万冰[2] 金戈[1] 张玉丽[3] 郭丽[1] 马铃铃 张梦月 

机构地区：[1]沈阳医学院基础医学院,沈阳110034 [2]沈阳医学院附属中心医院检验科,沈阳110024 [3]辽宁何氏医学院形态学教研室,沈阳110163

出　　处：《东北农业大学学报》2014年第12期31-35,共5页Journal of Northeast Agricultural University

基　　金：沈阳医学院科技基金项目(20133054)

摘　　要：研究黄连解毒汤（HLJDT）对肿瘤生长的抑制作用及其对肿瘤组织COX-2、VEGF、bFGF的影响。将健康雌性昆明种小鼠50只（体重18-22 g）随机分为对照组，环磷酰胺组（CTX），黄连解毒汤大、中、小剂量组（22.0、11.0、5.5 g·kg^-1）。通过皮下注射S180细胞悬液建立小鼠S180肉瘤模型，观察抑瘤率，并采用免疫组化的方法研究黄连解毒汤对肿瘤组织COX-2、VEGF、bFGF的作用。结果表明，黄连解毒汤大剂量组对小鼠S180肉瘤具有明显的抑制作用，免疫组化染色显示黄连解毒汤各剂量组对肿瘤组织中COX-2、bFGF表达有明显抑制作用，同时大、中剂量组对VEGF表达有明显抑制作用。说明黄连解毒汤对S180肉瘤具有抑制作用，其可抑制肿瘤组织中血管生成相关因子COX-2、VEGF、bFGF的表达，抑制肿瘤血管生长，抑制肿瘤生长，抗血管生成可能是其发挥治疗作用的另一种机制。The experiments were designed to study the effects of Huanglianjiedu Decoction（HLJDT） on antitumor activity in vivo and the expressions of COX-2, VEGF and bFGF in tumor tissues. 50 healthy female Kunming mice（18-22 g） were col ected, 50 tumor-bearing mice were randomly divided into NS control group, CTX positive group and 22.0, 11.0, 5.5 g·kg-1 HLJDT groups. We established S180 model of mice by transplanting 0.2 mL S180 cel suspension subcutaneously in mice, and immunohistochemical method was used to detect the expressions of COX-2, VEGF and bFGF.The results showed that the tumor-suppressing rate was higher in CTX group and HLJDT high dose group. COX-2 and bFGF in HLJDT groups and CTX group was lower than that in model group significantly. And the expressions of VEGF in HLJDT medium dose group and high dose group were markedly lower than that in model group. The results suggested that HLJDT had inhibitory effects on S180, and it also decreased the expression of COX-2, VEGF, bFGF in tumor tissue. Accordingly antiangiogenesis may be another mechanism for HLJDT to exert its treatment effects.

关 键 词：黄连解毒汤 S180 环氧合酶-2 血管内皮生长因子 碱性成纤维细胞生长因子 

分 类 号：R285[医药卫生—中药学]