犬毛色、毛色基因型及性别与导盲犬培训成功率的相关性研究  

Investigation of the Correlation of Coat Color,Genotypes of Coat Color and Sex with the Training Success Rate of Guide Dogs

作　　者：俞剑熊 张雅丽[1] 周子娟[1] 赵明媛[1] 韩芳[1] 王福金[1] 王爱国[1] 王靖宇[1] 

机构地区：[1]大连医科大学实验动物中心,大连116044

出　　处：《实验动物科学》2014年第6期32-35,共4页Laboratory Animal Science

基　　金：国家自然科学基金(No.31272392)

摘　　要：目的探究拉布拉多犬与金毛猎犬的毛色、毛色基因型及性别对导盲犬培训成功率的影响。方法采用PCR/测序方法对45只拉布拉多犬和16只金毛猎犬的毛色基因MC1R(c.C916T)、TYRP1(c.C991T)位点进行多态性检测,并对基因型加以分型,探讨犬毛色及毛色基因型与导盲犬培训成功率的相关性。另外对中国导盲犬大连培训基地犬只的性别进行统计分析,探讨犬性别与导盲犬培训成功率的关系。结果金毛猎犬共检测16只(14雄2雌),毛色表型均为金黄色,毛色基因型均为ee BB基因型;拉布拉多犬共检测45只(23雌22雄),毛色表型为黄色(23只)与黑色(22只),毛色基因型分别为ee BB、ee Bb、EEBb、Ee BB、Ee Bb。通过χ2检验的关联度分析,拉布拉多犬与金毛猎犬的毛色及毛色基因型与导盲犬培训成功率无显著相关性(P>0.05),另外,拉布拉多犬与金毛猎犬的性别与导盲犬成功率也无显著相关性(P>0.05)。结论拉布拉多犬和金毛猎犬的毛色、毛色基因型及性别与导盲犬成功率没有显著相关性,不能作为预测导盲犬培训成功率的有效指标。Objective To investigate the effect of coat color, genotypes of coat color and sex of Labrador retriever and Golden retriever on the training success rate of guide dogs. Method In order to analysis the correlation of the coat color or the genotypes of coat color with the training success rate of guide dogs, the polymorphism loci of coat color gene MC1R （c. C916T） and TYRP1 （c. C991T） were detected by using PCR/sequencing method, and then the genotypes were decided, for 45 Labrador retriever and 16 Golden retriever. In addition, the correlation between the sex and the training success rate of guide dogs was also analyzed by exploring the Guide dogs in China （Dalian） Guide dog training center. Result The genotype of 16 （14 male, 2 female） Golden retrievers （coat color： all gold） was all eeBB. The genotypes of 45 （22male, 23 female） Labrador retrievers （coat color： 23yellow, 22black） were eeBB, eeBb, EEBb, EeBB, EeBb, respectively. The results of chi-square correlation degree analysis showed that the coat color, and the genotypes of coat color, of Labrador retriever and Golden retriever had no significant correlation with the training success rate of guide dogs （P 〉 0.05 ）. In addition, the sex of Labrador retriever and Golden retriever also had no significant correlation with the training success rate of guide dogs （P 〉 0.05）. Conclusion The coat color, genotypes of coat color and sex have no significant correlation with the training success rate of guide dogs, and can not be used as the effective index to predict the training success rate of guide dogs.

关 键 词：拉布拉多犬 金毛猎犬 毛色基因型 导盲犬培训成功率 

分 类 号：Q38[生物学—遗传学]