瓦氏雅罗鱼群体基于CO Ⅰ序列的遗传多样性分析  

Genetic Diversity of Leuciscus waleckii Populations Based on CO Ⅰ Sequence

作　　者：窦新杰[1,2] 常玉梅[1] 唐然[1] 孙效文[1] 陶然[1,3] 王楠[1,2] 梁利群[1] 张丽霞 

机构地区：[1]中国水产科学研究院黑龙江水产研究所,淡水鱼类育种国家地方联合工程实验室,农业部淡水水产生物技术与遗传育种重点实验室,黑龙江哈尔滨150070 [2]上海海洋大学水产与生命学院,上海201306 [3]宁波大学海洋学院,浙江宁波315211 [4]内蒙古达拉特旗水产工作站,内蒙古鄂尔多斯014300

出　　处：《华北农学报》2014年第B12期29-34,共6页Acta Agriculturae Boreali-Sinica

基　　金：国家高技术研究发展计划项目(2011AA100402-3);黑龙江省科技攻关项目(GC12B307)

摘　　要：利用mt DNA COⅠ基因片段(有效长度635 bp)对来自达里湖(DL)、岗更湖(GG)、呼伦湖(HL)、黑龙江(HLJ)、松花江(SHJ)和乌苏里江(WSL)6个地理群体共72个样品进行了遗传多样性和遗传结构分析。从碱基组成来看,6个地理群体在COⅠ区段上的A+T含量(52.37%)明显高于G+C含量(47.67%),符合脊椎动物的碱基组成特点;从各项遗传参数来看,达里湖流域2个群体(DL和GG)的遗传多样性低于黑龙江流域4个群体(HL、HLJ、SHJ、WSL);6个群体共检测到20个单倍型,并共享1个单倍型,达里湖流域群体共享1个单倍型,黑龙江流域群体共享1个单倍型。虽然达里湖流域和黑龙江流域各群体间遗传分化明显(P<0.05),遗传结构存在差异,但AMOVA检验不支持这种遗传差异;另外,群体间频繁的基因交流进一步证实,6个地理群体亲缘关系近,有共同的起源,尚未达到分化为不同的种或亚种的水平。To analyzing the genetic diversity and genetic structure of Leuciscus waleckii based on mt DNA COⅠgene（635 bp length）,six geographical populations（72 samples） were sampled from Dali Lake（DL）,Ganggeng Lake（GG）,Hulun Lake（HL）,Amur River（HLJ）,Songhua River（SHJ） and Wusuli River（WSL）.On the base composition,A ＋ T content（52.37%） was slightly higher than that of G ＋ C content（47.63%）,corresponding to the characteristics of nucleotide composition in the vertebrates;On the genetic parameters,the genetic diversities of Dali Basin populations（DL and GG） were lower than those of Amur Basin counterparts（HL,HLJ,SHJ and WSL）;There were 20 haplotypes,of one haplotype was shared by all the six populations,one shared by Dali Basin and one shared by Amur Basin.Despite there were significant population differentiation between Dali Basin and Amur Basin（P 〈0.05） as well as genetic structure,the analysis of AMOVA and the values of frequent gene exchange（Nm） both demonstrated that the six Leuciscus waleckii populations had very close relationship,probably derived from a common ancestor,and had not yet differentiated to different species or subspecies based on present evidence.

关 键 词：瓦氏雅罗鱼 COⅠ基因 遗传多样性 遗传分化 

分 类 号：Q78[生物学—分子生物学] Q311