动物组织中3种β-肾上腺受体激动剂残留的高效液相色谱荧光检测法  

Determination of three kinds of β-adrenoceptor agonist residues in animal tissues by HPLC-fluorescence detection method

作　　者：张仁诚[1] 李吉平[2] 郝镯[2] 刘林娜[2] 李树民[2] 李楠[2] 刘全[2] 周莉 

机构地区：[1]沈阳军区总医院,辽宁省沈阳市110840 [2]军事医学科学院军事兽医研究所 [3]沈阳军区联勤部疾病预防控制中心

出　　处：《职业与健康》2015年第3期322-325,共4页Occupation and Health

摘　　要：目的盐酸多巴胺、硫酸沙丁胺醇和盐酸莱克多巴胺是β-肾上腺素受体激动剂,该研究目的是建立一种用于这3种成分的检测方法,以防止人类食用含有该残留成分的畜产品引发食物中毒。方法实验选用SHIMADZU ODS C18反相色谱柱（150 mm×4.6 mm,5μm）,以含10%乙腈的0.02 mol/L磷酸二氢钾溶液（p H=2.50）为流动相A、含50%乙腈的0.02 mol/L磷酸二氢钾溶液（p H=2.50）为流动相B,在0~6 min内由A液变为B液进行梯度洗脱。激发波长（λex）为280 nm,发射波长（λem）为309 nm,对3种β-肾上腺素受体激动剂进行分离和检测。结果盐酸多巴胺、硫酸沙丁胺醇和盐酸莱克多巴胺分别在5~200、5~200和10~200 ng范围内呈良好的线性关系,其相关系数R2分别为0.999 3、0.999 9和0.999 2,其标准回收率分别为（101.50±3.42）%、（99.46±2.93）%和（101.83±4.12）%,变异系数为3.37%、2.95%和4.05%。样品的回收率分别为（60.74±1.05）%、（96.30±0.62）%和（91.02±1.28）%,变异系数分别为1.72%、0.64%和1.40%。盐酸多巴胺、硫酸沙丁胺醇和盐酸莱克多巴胺的最低检测限分别为0.1、0.125和5 ng。结论该方法简单,快速,准确,可用于实验室动物性食品中残留药物的常规检测。[Objective]The dopamine hydrochloride, salbutamol sulfate and ractopamine hydrochloride are β-adrenoceptor agonists.This study is aiming to establish a method for determination of these three components, to prevent the food poisoning caused by eating the animal products with the β-adrenoceptor agonist residues. [Methods]The samples were extracted and purified with SHIMADZU ODS C18 reversed phase chromatographic column（150 mm×4.6 mm,5 μm）. 10% potassium dihydrogen phosphate solution with 10% acetonitrile was mobile phase A（p H=2.50）,while 0.02 mol/L potassium dihydrogen phosphate solution with50% acetonitrile was mobile phase B（p H=2.50）,and the gradient elution was performed by mobile phase A converting to mobile phase B within 0-6 min. With the excitation wavelength（λex） of 280 nm and the emission wavelength（λem） of 309 nm,the samples were detected.[Results]The calibration curve of dopamine hydrochloride, salbutamol sulfate and ractopamine hydrochloride showed good linearity in the range of 5-200 ng（R2=0.9993）,5-200 ng（R2=0.999 9） and 10-200 ng（R2=0.999 2）,respectively. The standard recovery rate was respectively（101.50 ±3.42）%,（99.46 ±2.93）% and（101.83 ±4.12）%,while the variation coefficient was respectively 3.37%,2.95% and 4.05%. The sample recovery rate was respectively（60.74 ±1.05）%,（96.30±0.62）% and（91.02±1.28）%,while the variation coefficient was respectively 1.72%,0.64% and 1.40%. The lowest detectable limit of dopamine hydrochloride,salbutamol sulfate and ractopamine hydrochloride was respectively 0.1ng, 0.125 ng and 5 ng. [Conclusion]This method is simple,rapid and accurate. It can be used as a conventional laboratory method to detect the drug residues in animal food.

关 键 词：HPLC 盐酸多巴胺 硫酸沙丁胺醇 盐酸莱克多巴胺 荧光检测 

分 类 号：R115[医药卫生—公共卫生与预防医学]