苏州市部分住院儿童感染性腹泻的病原学研究  

Etiological study of infectious diarrhea among some hospitalized children in Suzhou City

作　　者：李英杰[1] 汤全英 张梦寒 杨波 傅春玲[1] 陈学良 

机构地区：[1]苏州大学医学部公共卫生学院,江苏省苏州市215123 [2]苏州市疾病预防与控制中心

出　　处：《职业与健康》2015年第3期358-361,共4页Occupation and Health

基　　金：江苏省卫生厅预防医学科研课题资助项目(项目编号Y2012048)

摘　　要：目的分析苏州部分腹泻患儿的病原体检测结果,总结其病原构成及分布情况。方法共采集2013年6—11月苏州大学附属儿童医院腹泻患儿的粪便样本209份,采用实时荧光聚合酶链反应(PCR)方法检测艰难梭菌、分离培养其他常规病原菌,并采用酶联免疫吸附试验(ELISA)检测轮状病毒,PCR方法检测杯状病毒、星状病毒、腺病毒。结果 209例患儿中共127例(60.8%)检出病原体,其中30例(23.6%)存在合并感染。病毒感染中轮状病毒(64/118,54.2%)居于首位,其次为杯状病毒中的诺如病毒(39/118,33.1%)。细菌感染中艰难梭菌(32/44,72.7%)比例最高。合并感染以轮状病毒合并杯状病毒及轮状病毒合并艰难梭菌(8/30,26.7%)居多。病原体在性别间分布无差异,年龄分布集中于6月龄以上患儿。结论儿童腹泻的主要病原是轮状病毒、杯状病毒、艰难梭菌及沙门菌,且不同病原体的合并感染率较高,其中6月龄以上婴幼儿应是重点监测人群。[Objective]To analyze the detection results of the isolated pathogens from children with diarrhea in Suzhou City,summarize the composition and distribution of pathogens. [Methods]209 fecal samples were collected from children with diarrhea in the Children＇s Hospital Affiliated to Soochow University between June and November 2013. Real-time PCR assays were performed to detect Clostridium difficile. Other pathogenic bacteria were isolated and identified. The enzyme-linked immunosorbent assay was used to detect rotavirus. Calicivirus,astrovirus and adenovirus were tested by PCR. [Results]The pathogens were detected in 127（60.8%） of 209 children,and the concurrent infection was identified in 30 cases（23.6%）.Rotavirus（64/118,54.2%）was the most common virus isolated from fecal samples,followed by the norovirus of calicivirus（39/118,33.1%）. The most frequent bacterial pathogen was Clostridium difficile（32/44,72.7%）. The concurrent infection mainly caused by rotavirus-calicivirus and rotavirus-Clostridium difficile（8/30,26.7%）. There was no difference in distribution of pathogens between boys and girls. The majority of cases were children over 6 months old. [Conclusion]The main pathogens of infectious diarrhea in children are rotavirus, calicivirus, Clostridium difficile and Salmonella, and the rate of concurrent infection with different pathogens is high. Children over 6 months old are the key population of monitoring.

关 键 词：腹泻 病原学 合并感染 儿童 

分 类 号：R181.3[医药卫生—流行病学]