糖浓度波动对单核细胞表达CD11b、TNF-α的影响  

Effect of glucose fluctuation on CD11b and TNF-α produced by monocytes

作　　者：宋华静[1] 寇伟[1] 蒋雪羚[1] 李娟[1] 亓文波[2] 

机构地区：[1]山东省泰山疗养院,山东泰安271000 [2]泰山医学院附属泰安医院

出　　处：《山东医药》2014年第9期28-30,共3页Shandong Medical Journal

基　　金：泰安市科学技术局资助课题(20113047)

摘　　要：目的探讨葡萄糖浓度波动对单核细胞表达CD11b及肿瘤坏死因子-α(TNF-α)的影响。方法根据葡萄糖浓度将THP-1细胞株随机分为对照组、高葡萄糖组、葡萄糖浓度波动组和渗透压控制组。采用流式细胞仪检测单核细胞表面CD11b表达,酶联免疫法测定TNF-α浓度,并进行各组间的比较。结果高葡萄糖组CD11b、TNF-α浓度高于对照组,葡萄糖浓度波动组高于高葡萄糖组,渗透压控制组介于高葡萄糖组和葡萄糖浓度波动组之间(P均<0.05)。结论葡萄糖浓度波动较单纯高糖更能促进单核细胞分泌CD11b、TNF-α。Objective To investigate the effectss of fluctuation of glucose concentrations on CD11b and tumor necrosis factor（ TNF）-α produced by Mmonocytes,and discuss the role of the blood glucose in the pathogenesis of diabetic macroangiopathy.Methods The THP-1 cell strain was randomly divided into the control group,high glucose group,fluctuating glucose concentration group and osmotic pressure control group.The expression of CD11b on the surface of monocytes was detected by flow cytometry.The concentrations of TNF-α were detected by ELISA and then were compared in each group.Results The concentrations of TNF-α and CD11b in the high glucose group were significantly higher than those in the control group,the levels of TNF-α and CD11b of the fluctuating glucose group were higher than those of the high glucose group,and the concentrations of TNF-α and CD11b of the osmotic pressure control group were between those of the high glucose group and the fluctuating glucose group.The differences between groups was statistically significant（ all P〈 0.05）.Conclusion The fluctuation of glucose can promote monocytes secreting more CD11b and TNF-α,which can produce a greater cellular toxicity than simple high glucose does.

关 键 词：单核细胞 葡萄糖 巨噬细胞1抗原 肿瘤坏死因子 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]