亚硒酸钠对海洛因吸食者T淋巴细胞增殖及其线粒体功能的影响  

Effects of sodium selenite on T lymphocyte proliferation and mitochondrial function in vitro

作　　者：付青梅[1] 张永春[1] 张永创[1] 卜英博 谢淼[1] 王茜[1] 

机构地区：[1]武警贵州总队医院,贵阳550005

出　　处：《武警医学》2015年第2期175-179,182,共6页Medical Journal of the Chinese People's Armed Police Force

基　　金：贵州省科技术基金项目(黔科合J字[2012]2322号)

摘　　要：目的探讨亚硒酸钠对海洛因吸食者T淋巴细胞的保护作用及其与线粒体解偶联相关指标的关系。方法使用不同海洛因吸食年限人员外周血,提取T淋巴细胞;四甲基偶氮唑蓝(methyl thiazolyl tetrazolium,MTT)法检测细胞生长情况,荧光分光光度法检测细胞内活性氧(reactive oxygen species,ROS)、细胞线粒体膜电位(mitochondrial membrane potential,△Ψm),用紫外分光光度计测线粒体通透性转运孔(mitochondrial permeability transition pore,MPTP)。结果随着吸食海洛因的时间增加,T淋巴细胞的增殖活性下降,差异有统计学意义(P<0.05);随着海洛因吸食年限的增加,T淋巴细胞线粒体MPTP增加、△Ψm下降、ROS升高(P<0.05);亚硒酸钠可逆转上述改变,且存在量效关系(P<0.05)。结论长时间吸食海洛因所造成的氧化应激状态,通过线粒体途径对T淋巴细胞的功能和增殖情况有不利影响;亚硒酸钠可以通过其抗氧化作用,降低ROS水平来逆转上述过程,对T淋巴细胞具有保护作用。Objective To study whether or not there is protective effect of sodium selenite on T lymphocytes of heroin addicts,and if it correlates with mitochondrial uncoupling related indicators. Methods T lymphocytes extracted from peripheral blood of people who abuse heroin in different periods. Cell proliferation was detected by MTT,the levels of ROS,△Ψm were detected by fluorescence spectrophotometer,MPTP was measured by ultraviolet spectrophotometer. Results With the increased duration of heroin-abuse,T lymphocyte proliferation activity decreased significantly（ P 0. 05）; T lymphocyte mitochondrial MPTP increased,△Ψm decreased,ROS increased（ P 0. 05）. Sodium selenite can reverse these changes,and there is a dose-effect relationship（ P 0. 05）. Conclusions Oxidative stress caused by prolonged heroin abuse has an adverse effect on the function and proliferation of T lymphocytes via the mitochondrial pathway. Sodium selenite has a protective effect on T lymphocytes by its antioxidant effect,which reduces the level of ROS to reverse the above process.

关 键 词：亚硒酸钠 T淋巴细胞 线粒体 线粒体通透性转运孔 活性氧 细胞线粒体膜电位 

分 类 号：R392.12[医药卫生—免疫学]