过氧化氢处理对Ⅰ型胶原结构和性能的影响  

Effect of Hydrogen Peroxide Treatment on Structure and Performance of Type Ⅰ Collagen

作　　者：龚居霞 刘新华[1,2] 但卫华[1,2] 刘科[1,2] 路翠娟[1,2] 但年华[1,2] 

机构地区：[1]四川大学皮革化学与工程教育部重点实验室,四川成都610065 [2]四川大学生物医学工程技术研究中心,四川成都610065

出　　处：《中国皮革》2015年第1期10-13,23,共5页China Leather

基　　金：四川大学-德国司马公司创新奖学金(基金)科研项目;四川大学大学生创新创业训练计划;项目编号:20141294

摘　　要：采用不同浓度的过氧化氢对Ⅰ型胶原进行处理,并通过红外、DSC、电泳、SEM、AFM和特性黏度检测,对过氧化氢处理胶原后胶原结构的变化进行了探究分析。结果表明:过氧化氢与胶原可发生化学交联,AFM检测证实了当过氧化氢浓度增加时胶原分子间距变小,纤维间缠绕紧密,尤其是当浓度增为2%时,胶原分子更是缠结在一起,形成更为粗大的纤维束,这使得胶原的热稳定性、特性黏度、分子质量得到不同程度地提高;但当过氧化氢浓度达到3%时,其反而对胶原结构有一定破坏,使胶原产生了一定的降解。研究认为,过氧化氢对胶原起着交联和降解的双重作用:在低浓度时对胶原的作用以交联为主,在高浓度时对胶原的作用以降解为主。The type I collagen was modified by different concentrations of hydrogen peroxide.The changes of collagen structure after modified were analyzed by IR,DSC,EP,SEM,AFM and intrinsic viscosity.The results show that chemical crosslinking occurs between hydrogen peroxide and collagen.AFM tests confirm that the collagen molecular space decreases with the concentration of hydrogen peroxide increasing,and fiber winding is compact.Especially when the concentration increases to 2%,collagen molecules are entangled together to form a more bulky fiber bundle,which makes the thermal stability,intrinsic viscosity and molecular weight of collagen increase in different degree.But when the hydrogen peroxide concentration reach 3%,it can damage the structure of collagen and have a certain degradation effect on collagen.In conclusion,hydrogen peroxide plays a dual role of crosslinking and degradation to collagen;the main effect to collagen is crosslinking at low concentration and degradation at high concentration.

关 键 词：Ⅰ型胶原 过氧化氢 结构检测 性能分析 

分 类 号：TS51[轻工技术与工程—皮革化学与工程]