脑红蛋白在成年西门塔尔牛几种主要器官组织中的分布  

Distribution of neuroglobin in several major organs and tissues of the adult simmental

作　　者：高雪晋 刘霞[1] 杨阳 梁春花 杜晓华 

机构地区：[1]甘肃农业大学生命科学技术学院 [2]动物医学院,兰州730070

出　　处：《解剖学报》2015年第1期32-37,共6页Acta Anatomica Sinica

基　　金：甘肃省高等学校基本科研业务费资助项目(2013);甘肃省教育厅研究生导师资助项目(1102-04)

摘　　要：目的探寻脑红蛋白(NGB)在成年西门塔尔牛不同组织细胞的分布特征。方法选择健康成年西门塔尔牛5只,利用免疫组织化学染色SP法观察NGB在其不同组织细胞的分布特征。结果结果显示,NGB在成年西门塔尔牛大脑和小脑皮质部、嗅球,海马,脊髓、肾上腺皮质部与髓质部、垂体、松果体、胰、睾丸、附睾、卵巢、子宫、胃、肠、肝、颌下腺、腮腺、心肌、脾脏、肺脏和肾脏等组织细胞中均有表达,NGB免疫阳性物质主要定位于细胞质。此外,本研究发现NGB在西门塔尔牛十二指肠段小肠腺细胞的表达强度要高于十二指肠腺细胞。结论NGB在成年西门塔尔牛多种器官组织细胞中的广泛分布,提示NGB不仅在氧的利用及功能活动中发挥作用,在其它生理活动中可能也担负着重要的功能。Objective To explore the localization of neuroglobin （NGB） in adult simmental tissues and cells. Methods Five health adult simmentals were used in this study. NGB expression in adult simmental tissues and cells was localized using the immunohistochemieal SP technique. Results NGB-positive cells were distributed in tissues and cells of the cerebral and cercbellar cortex, olfactory bulb, hippocampus, spinal cord, adrenal cortex and medulla, hypophysis, pineal body, pancreas, testis, epididymis, ovary, uterus, stomach, intestines, liver, submandibular gland, parotid gland, cardiac muscle, spleen, lung and kidney, NGB-immunoreactive product was mainly located in the cytoplasm of these cells. In addition, the expression of NGB-positive in duodenum intestinal crypt cells was higher than that the expression of glandulae duodenales cells in simmental. Conclusion The widely expression of NGB in various organs, tissues and cells of the adult simmental suggest that NGB not only plays a role in the utilization of oxygen and physiological functions, there may have other important functions.

关 键 词：脑红蛋白 分布 免疫组织化学 西门塔尔牛 

分 类 号：Q786[生物学—分子生物学]