法舒地尔对阻塞性黄疸大鼠肝缺血再灌注损伤的影响  

Fasudil protects from hepatic ischemia reperfusion injury in rats model of obstructive jaundice

作　　者：朱耿隆[1] 蔡潮农[1] 贾英斌[1] 洪晓鹏[1] 张百萌[1] 

机构地区：[1]中山大学附属第五医院普通外科,珠海519000

出　　处：《中华肝胆外科杂志》2015年第1期44-48,共5页Chinese Journal of Hepatobiliary Surgery

基　　金：广东省医学科研基金立项课题（A2012209）;珠海市科技局医药卫生重大项目（PB20081002）

摘　　要：目的 探讨法舒地尔对阻塞性黄疸大鼠肝缺血再灌注损伤的影响.方法 成熟SD大鼠160只随机分成实验组（A组）及对照组（B组）两大组,两组再分别分为4个亚组,分别为假手术组（A1、B1组）、阻塞性黄疸组（A2、B2组）、肝缺血再灌注组（A3、B3组）及阻塞性黄疸＋肝缺血再灌注组（A4、B4组）.A2、B2组采用双重结扎切断胆总管的方法建立模型,A3、B3组采用阻断肝门部血管30 min后再灌注的方法建立模型.A4、B4组于建立阻塞性黄疸模型1周后再次建立缺血再灌注模型.A组于缺血前30 min腹腔内注射法舒地尔10 mg/kg,B组对应注射等量生理盐水.两组分别于再灌注0h、1h、2h、6h取材,检测血清肝功能改变及血清内皮素1水平.采用SPSS软件对重复测量结果进行统计分析,光镜下观察肝脏病理学改变.结果 实验组各时点血清转氨酶水平、血清内皮素1水平均显著低于对照组（P＜0.05）,实验组各时点光镜下肝脏病理组织学损伤也较对照组明显减轻.结论 法舒地尔对阻塞性黄疸大鼠的肝缺血再灌注损伤具有保护作用.Objetive To investigate the effect of Fasudil on the hepatic ischemia repeffusion injury on rats of obstructive jaundice.Methods 160 mature SD rats were randomly assigned into 2 large groups,namely experimental group （Group A） and control group （Group B）.While each large group was subdivided into 4 subgroups,including sham operation subgroups（Group A1 and B1）,obstructive jaundice model subgroups（Group A2 and B2）,ischemia reperfusion model subgroups （Group A3 and B3）and obstructive jaundice plus ischemia reperfusion model subgroups （Group A4 and B4）.In Group A2 and B2,obstructive jaundice models were made on rats by double ligating and cutting the common bile ducts.In Group A3 and B3,the ischemia reperfusion injury models were built by blocking the hepatic portal vessel for 30 minutes before reperfusion.In Group A4 and B4,the ischemia reperfusion injury models were built in 1 week after building the obstructive jaundice models.For Group A,10 mg/kg fasudil was intraperitoneally injected at 30 minutes before ischemia.For Group B,equal amount of physiological saline was injected.Samples were gained on zero hour,1 hour,2 hour and 6 hour post reperfusion.The serum indexes for liver function and serum Endothelin 1 level were tested and analyzed through SPSS.Pathological changes of liver were viewed correspondingly.Results Compared to control groups （Group Bs）,experimental groups （Groups As） have better liver function,lower Endothelin 1 level （P 〈 0.05）,as well as less severe microscopic histological damage.Conclusion Fasudil can effectively protect rats of obstructive jaundice from hepatic ischemia reperfusion injury.

关 键 词：法舒地尔 阻塞性黄疸 缺血再灌注损伤 

分 类 号：R657.3[医药卫生—外科学]