小鼠内皮型一氧化氮合酶表达的变化与肾间质纤维化的关系  

Relationship between change of endothelial nitric oxide synthase expression and renal interstitial fibrosis in mice

作　　者：孙东[1,2] 麻艳艳[2] 王亚芬[2] 尹忠诚[2] 周旭东 李晓菊 肖爱国 

机构地区：[1]伊犁哈萨克自治州奎屯医院肾脏病科,新疆维吾尔自治区833200 [2]徐州医学院附属医院肾脏病科

出　　处：《江苏医药》2015年第2期132-134,F0002,共4页Jiangsu Medical Journal

基　　金：国家自然科学基金(81270769);江苏省医学重点人才项目(RC2011116);江苏省第七批"六大人才高峰"资助项目(2010-ws043);江苏省青蓝工程资助项目(53051106);新疆伊犁哈萨克自治州奎屯市课题(201134);徐州市科技发展基金(XF10C076);徐州医学院院长专项基金(2010KJZ23)

摘　　要：目的探讨内皮型一氧化氮合酶(eNOS)在小鼠单侧输尿管梗阻(UUO)模型肾组织中的变化及其与肾间质纤维化的关系。方法 48只小鼠随机均分为UUO模型组和假手术组,每组术后第1、3、7、14天分别处死6只小鼠,观察肾组织的病理改变,检测eNOS和结缔组织生长因子(CTGF)表达,计算管周毛细血管(PTC)密度。结果模型组术后第7天部分肾小管萎缩,纤维化形成;术后第14天肾小管间质纤维化更加明显。假手术组eNOS表达较多,CTGF低表达。与假手术组相比,模型组第7、14天eNOS表达减少(P<0.05),CTGF表达增多(P<0.05),第3-14天PTC密度下降(P<0.05)。结论小鼠UUO肾组织eNOS表达下降参与了肾间质纤维化的进展。Objective To investigate the change of endothelial nitric oxide synthase（eNOS）expression in renal tissues of mice with unilateral ureteral occlusion（UUO）and its relationship with renal interstitial fibrosis.Methods Forty-eight mice were equally randomized into 2groups of A（UUO model）and B（sham operated）.Six mice from each group were killed on the 1^st,3^rd,7^th and 14^th day after operation,respectively,for observing the change of renal pathology,calculating the density of peritubular capillaries（PTC）and detecting the expressions of eNOS and connective tissue growth factor（CTGF）.Results Some renal tubulars were atrophied and renal interstitial fibrosis formed on the 7^th day,which were more obvious on the 14^th day in group A.The expression of eNOS was more,while that of CTGF was less in group B.Compared with group B,the expression of eNOS was decreased and that of CTGF was increased on the 7^th and 14^th day（P〈0.05）,and PTC density was decreased from the 3^rd day to 14^th day in group A（P〈0.05）.Conclusion The decreased expression of eNOS in renal tissues of UUO mice is involved in the development of renal interstitial fibrosis.

关 键 词：内皮型一氧化氮合酶 肾间质纤维化 

分 类 号：R692[医药卫生—泌尿科学]