前胡丙素对大鼠缺血-再灌注心肌肌浆网钙泵功能及其蛋白水平的影响  

Effects of praeruptorin C on function and expression of sarcoplasmic reticulum Ca^(2+)-ATPase in isolated ischemia-reperfusion hearts in rats

作　　者：王金桂[1] 祝宝华[2] 于晓頔[2] 陈开祥[2] 奚群英[2] 刘晨[2] 

机构地区：[1]无锡市中医医院心血管病科,江苏省214037 [2]东南大学附属扬州医院心血管病研究所

出　　处：《江苏医药》2015年第2期138-141,共4页Jiangsu Medical Journal

基　　金：江苏省135工程医学重点人才课题资助项目(RC2002016)

摘　　要：目的研究前胡丙素对大鼠缺血-再灌注(I-R)心肌肌浆网钙泵(SERCA)的影响及机制。方法 24只雄性SD大鼠随机均分为正常对照组、I-R组、前胡丙素+I-R组和聚乙二醇400+I-R组。检测再灌注15min时冠状动脉流出量(CF)和冠状动脉流出液中乳酸脱氢酶(LDH)漏出量,采用改良对硝基苯酚棕榈酸酯(p-NPP)法和Millipore过滤技术测定SERCA的活性和三磷酸腺苷依赖的SERCA45 Ca2+摄取率,Western blot法检测心肌细胞SERCA蛋白表达水平。结果与I-R组相比,前胡丙素+I-R组CF、SERCA蛋白表达量、SERCA的活性和45 Ca2+摄取率增高(P<0.01),LDH漏出量降低(P<0.01)。结论前胡丙素对离体大鼠心脏I-R损伤有保护作用,可能与其减轻I-R损伤引起的心肌SERCA功能和蛋白表达抑制有关。Objective To investigate the effect and underlying mechanism of praeruptorin C（Pra-C）on sarcoplasmic reticulum Ca^2＋-ATPase（SERCA）in isolated ischemia-reperfusion（I-R）hearts in rats.Methods Twenty-four male SD rats were equally and randomly divided into 4groups of A（normal control）,B（myocardial I-R injury）,C（pretreated with Pra-C before myocardial I-R injury）and D（pretreated with polyethylene glycol 400 before myocardial I-R injury）.At 15 min after reperfusion,coronary flux（CF）and LDH were examined and the SERCA activity and ^45 Ca^2＋ uptake were evaluated by modified p-NPP and Millipore filtering technique.The expression of SERCA protein was detected by Western blot.Results Compared with group B,CF,protein expression level and activity of SERCA and ^45 Ca^2＋ uptake in group C were significantly increased（P〈0.01）,while LDH was significantly decreased（P〈0.01）.Conclusion Pra-C has a significant protective effect on I-R injury of isolated rat hearts,which could be associated with alleviating the inhibition of protein expression and function of SERCA induced by I-R injury.

关 键 词：缺血-再灌注损伤 前胡丙素 肌浆网钙泵 

分 类 号：R541[医药卫生—心血管疾病]