外源性激素对异种移植后人胎卵巢组织的影响  

Effect of exogenous gonadotrophin on human fetal ovarian tissue after xenotransplantation

作　　者：童月红[1] 周颖[2] 吴丹丹[2] 傅晓艳 

机构地区：[1]金华市中心医院妇产科,浙江金华321000 [2]温州医科大学附属第一医院生殖医学中心,浙江温州325000 [3]金华市职业技术学院解剖学教研室,浙江金华321000

出　　处：《温州医学院学报》2015年第1期10-13,共4页Journal of Wenzhou Medical College

基　　金：国家自然科学基金资助项目(30370749)

摘　　要：目的：探讨外源性促性腺激素对异种移植后的冻融人胎卵巢组织卵泡发育的影响。方法：将冻融人胎卵巢组织移植到裸鼠肾被膜下，裸鼠随机分为A、B、C 3组，分别以不同方式给予外源性促性腺激素—孕马血清促性腺激素（PMSG），并分别于术后10～14周、18～22周、26～30周回收移植物，行组织学评价和增殖细胞核抗原（PCNA）免疫组织化学分析。结果：10～14周和18～22周各组的原始、初级、次级卵泡数之间差异均无统计学意义（P＞0.05）。26～30周，A、B组次级卵泡数显著高于C组，差异均有统计学意义（P＜0.05）。各组均可见窦卵泡发育。结论：冻融人胎卵巢组织异种移植于雌性去势裸鼠后，无外源性激素作用环境下卵泡也可发育至窦卵泡；而不同方式的外源性促性腺激素作用均可进一步促进组织中卵泡的发育。Objective： To investigate the effect of exogenous gonadotrophin on the follicular development of cryopreserved human fetal ovarian tissue after xenotransplantation.Methods： The cryopreserved fetal ovarian tissues were grafted under kidney capsules of the nude mice. Mice randomly assigned to three different groups （A, B, C）, and were treated with PMSG by different ways. Grafts of each group were recovered in batches at 10-14 weeks, 18-22 weeks, 26-30 weeks after operation. Grafts were evaluated using conventional histology and detected proliferating core antigen （PCNA） using immunohistochemical method.Results： There was no signiif-cant difference among three groups of preantral follicles. At 26-30 weeks, the numbers of secondary follicles in group C were signiifcantly less than that in group A and B （P＆lt;0.05）. Antral follicles were discovered in each group.Conclusion： The follices of the cryopreserved human fetal ovarian tissue can develop to antral follicle without exogenous hormone after xenotransplantation, and follicles can be promoted by exogenous gonadotropin.

关 键 词：人胎卵巢 异种移植 促性腺激素 

分 类 号：R318.52[医药卫生—生物医学工程]