胰高血糖素样肽-1对高脂饲养大鼠卵巢生物钟节律的影响  

Effect of Glucagon-like peptide-1 on ovarian circadian clock rhythm of rats fed with high fat diet

作　　者：姚志娜 韩婷[1] 范秀玲[1] 章由生[1] 宋佳伦[1] 

机构地区：[1]山东大学附属生殖医院妇科,山东济南250021

出　　处：《山东大学学报（医学版）》2015年第2期19-21,33,共4页Journal of Shandong University:Health Sciences

摘　　要：目的观察胰高血糖素样肽-1(GLP-1)对高脂饲养大鼠受损卵巢生物钟的治疗作用。方法 Wistar大鼠随机分为普通饲养组、高脂饲养组及高脂饲养+GLP-1干预组;8周后测空腹血糖、胰岛素、胆固醇、甘油三酯、雌二醇、卵泡刺激素、黄体生成素的水平;实时定量PCR检测卵巢相关基因及生物钟基因的表达。结果 GLP-1对高脂饲养大鼠血清生化指标产生一定影响。与普通饲养组相比,高脂饲养组大鼠始基卵泡募集因子抗苗勒氏管激素(AMH)、生长分化因子-9(GDF-9)的表达水平降低(P<0.05),而高脂饲养GLP-1干预组AMH、GDF-9的mRNA的表达明显改善。此外,与普通饲养组相比,高脂饲养组大鼠卵巢生物钟相关基因的表达具有明显变化,而GLP-1可改善高脂饲养对卵巢生物钟相关基因表达的影响。结论 GLP-1对高脂饲养所致的卵巢生物钟受损具有改善作用。Objective To explore the therapeutic effect of glucagon-like peptide-1（GLP-1）on impaired circadian gene of ovary in rats fed with high-fat diet.Methods Wistar rats were randomly divided into control group,high-fat diet group and GLP-1 intervention group.Eight weeks later,the levels of fasting glucose,insulin,cholesterol,triglyceride, E2,FSH and LH were tested.The expression of ovary related gene and circadian gene in ovary tissues were detected with Real-time PCR.Results GLP-1 had certain effect on serum biochemical indexes in rats fed with high fat.Real-time PCR results showed that,compared with control group,the levels of primordial follicle recruitment factor anti-mul-lerian homone （AMH）and growth differentiation factor （GDF-9）were significantly decreased in rats fed with high-fat diet （P 〈0.05）.However,the expression of AMH and GDF-9 mRNA in high-fat diet rats treated with GLP-1 were markedly elevated.Besides,compared with control group,the expression of ovary circadian clock related genes was affected in rats fed with high-fat diet,and GLP-1 could improve the expression of ovary circadian gene.Conclusion GLP-1 could ameliorate the expression of ovary circadian gene in rats fed with high-fat diet.

关 键 词：胰高血糖素样肽-1 生物钟 卵巢 高脂饲养 大鼠 

分 类 号：R711.6[医药卫生—妇产科学]