外源AtCBF3基因导入对苹果组培苗耐盐性的影响  

Effect of the Exogenous At CBF3 Gene Introduction on Salt Tolerance in Apple in vitro Plantlets

作　　者：李银峰[1] 田海青[1] 师校欣[1] 杜国强[1] 

机构地区：[1]河北农业大学园艺学院,保定071001

出　　处：《分子植物育种》2015年第1期145-149,共5页Molecular Plant Breeding

基　　金：河北省自然科学基金项目(C2012204091;C2014204115)资助

摘　　要：本研究以转At CBF3基因苹果嘎拉组培苗为试验材料,采用不同浓度的Na Cl为胁迫因子,探讨外源基因导入对苹果组培苗耐盐性的影响。研究结果表明:转基因苹果组培苗受害指数随着盐浓度的升高而增大,生根率随Na Cl浓度的升高而降低。在4 g/L Na Cl胁迫下,随胁迫时间的延长组培苗的受害指数呈上升趋势,在胁迫8 d时,转基因株系的受害指数显著低于非转基因株系。Na Cl胁迫明显抑制苹果组培苗不定根发生,转基因株系与非转基因株系间差异不显著。Na Cl胁迫下转基因株系的相对电导率显著低于非转基因株系。研究结果说明外源At CBF3基因的导入一定程度上提高了苹果组培苗的耐盐性,然而对其不定根发生未产生显著影响。Transgenic lines of Gala apple plantlets carrying exogenous A tCBF3 gene were inoculated on media supplying with different concentration of NaC1 as stress factor to evaluate their tolerance to salt. The results showed that the plantlets injury index increased and the rooting rate decreased along with the increase of NaCI supplying in the media. Under the stress of 4 g/L NaC1, the plantlets injury index increased with elongation of the stress treatment, and there was a significant difference found between the transgenic lines and the non transgenic control on the eighth day of the stress. The adventitious root generation from the plantlets was significantly inhibited by the NaC1 stress, whereas there was no difference found between the transgenic lines and the control. The relative conductance of the transgenic lines was significant lower compared to that of the non transgenic control under the NaC1 stress. It could be proposed that the transgenic apple harbouring exogenous A tCBF3 gene might increase the salt tolerance in plant growth in some extent, but it seems no effect on its adventitious root regeneration.

关 键 词：苹果 AtCBF3基因 组培苗 耐盐性 相对电导率 不定根 

分 类 号：S661.1[农业科学—果树学]